Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54157 МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ In the genome of catfish Ictalurus Punctatus discoered new gene encoding an enzyme LDH/MDH superfamily В геноме сома Ictalurus Punctatus обнаружен новый ген, кодирующий фермент суперсемейства ЛДГ/МДГ Пузакова Л В Puzakova L V kvluda@yandex.ru Пузаков М В Puzakov M V Институт морских биологических исследований им. А.О. Ковалевского РАН ru A.O. Kovalevsky Institute of Marine Biological Research of RAS ru Институт морских биологических исследований им. А.О. Ковалевского РАН ru A.O. Kovalevsky Institute of Marine Biological Research of RAS ru 25 06 2017 25 06 2017 2 1 221 225 20 06 2017 20 06 2017 https://rusjbpc.ru/en/nauka/article/54157/view

При in silico исследовании генов, кодирующих ферменты из суперсемейства ЛДГ/МДГ у различных организмов, мы обнаружили, что аминокислотная последовательность канального сома Ictalurus punctatus , кодируемая геном Mdh1 , значительно отличается от продуктов генов Mdh1 других животных. Обнаруженная последовательность имеет консервативный домен, характерный для ферментов суперсемейства ЛДГ/МДГ, однако ни с одним из ферментов этого суперсемейства существенного сходства у нее нет. Таким образом, нами обнаружен ген, кодирующий новый фермент из суперсемейства ЛДГ/МДГ, который мы обозначили как unLdh/Mdh (unknown Ldh/Mdh) .

In silico studies of genes encoding enzymes from LDH/MDH superfamily in different organisms, we found that the aminoacid sequence of the channel catfish Ictalurus punctatus,encoded by the Mdh1 gene, is significantly different from the products of the Mdh1 genes other animals. The detected sequence has a conservative domain specified for the enzymes of the LDH/MDH superfamily, however, it has no significant similarity to any enzyme of this superfamily. Thus, we detected a gene encoding a new enzyme from the LDH/MDH superfamily, which we designated as unLdh/Mdh (unknown Ldh/Mdh).

 лактатдегидрогеназа малатдегидрогеназа ЛДГ МДГ энергетический обмен malate dehydrogenase lactate dehydrogenase LDH MDH metabolism

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