Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54181 МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ Translational diffusion of proteins in highly concentrated solutions Трансляционная диффузия белков в высококонцентрированных растворах Кусова А М Kusova A M alexakusova@mail.ru Ситницкий А Э Sinitsky A E Идиятуллин Б З Idiyatullin B Z Бакирова Д Р Bakirova D R Зуев Ю Ф Zuev Yu F Казанский институт биохимии и биофизики Казанского научного центра РАН; Казанский (Приволжский) Федеральный Университет ru Kazan Institute of Biochemistry and Biophysics, Russian Academy of Scien; Kazan (Volga Region) Federal University ru Казанский институт биохимии и биофизики Казанского научного центра РАН ru Kazan Institute of Biochemistry and Biophysics, Russian Academy of Scien ru Казанский институт биохимии и биофизики Казанского научного центра РАН ru Kazan Institute of Biochemistry and Biophysics, Russian Academy of Scien ru Казанский институт биохимии и биофизики Казанского научного центра РАН ru Kazan Institute of Biochemistry and Biophysics, Russian Academy of Scien ru Казанский институт биохимии и биофизики Казанского научного центра РАН; Казанский (Приволжский) Федеральный Университет ru Kazan Institute of Biochemistry and Biophysics, Russian Academy of Scien; Kazan (Volga Region) Federal University ru 25 06 2017 25 06 2017 2 1 317 321 20 06 2017 20 06 2017 https://rusjbpc.ru/en/nauka/article/54181/view

В настоящем исследовании основное внимание уделяется получению обобщенной концентрационной зависимости коэффициента самодиффузии (КСД) трипсина и α - химотрипсина в качестве представителей глобулярных сфероидальных белков и фибриногена в качестве примера белка сложного строения, имеющего сильно вытянутую форму с глобулярными и неструктурированными участками. Эксперименты проводились методом ядерного магнитного резонанса (ЯМР) с использованием импульсного градиента магнитного поля (ИГМП). Полученная обобщенная концентрационная зависимость КСД белков проанализирована в рамках феноменологического подхода, основанного на фрикционном формализме неравновесной термодинамики. Показана возможность использования данного феноменологического подхода для белков различной формы и размеров в широком диапазоне концентраций. Определены динамические параметры системы, а именно: коэффициенты трения между молекулами белка и между молекулами белка и растворителем. Предложено качественное объяснение полученных значений.

The present study focuses on obtaining a generalized concentration dependence of the self-diffusion coefficient for trypsin and α-chymotrypsin as representatives of globular spheroidal proteins and for fibrinogen as an example of irregular-shaped protein. Diffusion experiments were performed by nuclear magnetic resonance (NMR) using a pulsed magnetic field gradient (PFG). The resulting generalized concentration dependence of the self-diffusion coefficient of proteins was analyzed within the framework of known theoretical approach - phenomenological approach based on the frictional formalism of non-equilibrium thermodynamics. We showed the possibility of using the phenomenological approach to describe the mobility of proteins of various shapes and sizes over a wide range of concentrations. The dynamic parameters of the system were determined: friction coefficients between protein molecules and friction coefficients between protein and solvent molecules. A qualitative explanation of the obtained values is proposed.

 ЯМР диффузия белки концентрационная зависимость КСД

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