Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54185 МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ Formation of aggregates of crown-containing derivative phenoxason and their complexes with DNA molecule Формирование агрегатов краунсдержащего производного феноксазона и их комплексов с молекулой ДНК Морошкина Е Б Moroshkina E B evmorosh@mail.ru Седова О Б Sedova O B Санкт-Петербургский государственный университет ru Saint Petersburg State University ru Костромской государственный университет им. Н.А. Некрасова ru N.A. Nekrasov Kostroma State University ru 25 06 2017 25 06 2017 2 1 334 338 20 06 2017 20 06 2017 https://rusjbpc.ru/en/nauka/article/54185/view

Кинетика образования в водно-солевых растворах упорядоченных агрегационных структур краунсодержащим производным феноксазона (КФ), обладающим противоопухолевой активностью, и его взаимодействие с молекулой ДНК исследованы методами спектрофотометрии и спектрополяриметрии. Показано, что в зависимости от природы противоиона и ионной силы среды КФ образует агрегационные структуры Н- и J-типа в результате межмолекулярного взаимодействия радикалов -бензо-(15-краун-5), ассоциирующих катионы K+ или Na+. Высокое сродство КФ к ДНК в присутствии ионов K+ препятствует образованию агрегатов. В присутствии ионов Na+ процессы образования агрегатов КФ и его комплексов с ДНК идут параллельно. Наличие краунгруппировок в структуре КФ влияет на его способ связывания с ДНК в зависимости от природы противоиона в растворе.

Kinetics of the formation of ordered aggregation structures in aqueous-salt solutions of the crown-containing phenoxazone derivative (CPh), which has antitumor activity, and the interaction of the latter with the DNA molecule were studied by spectrophotometry and spectropolarimetry. It is shown that, depending on the nature of the counterion and the ionic strength of the medium, CPh forms H- and J-type aggregation structures as a result of the intermolecular interaction of -benzo-(15-crown-5) radicals associating K+ or Na+ cations. The high affinity of CPh to DNA in the presence of K+ ions prevents the formation of aggregates. In the presence of Na+ ions, the formation of complexes with DNA and CPh aggregates proceeds in parallel.The presence of crown-radicals in the CPh structure affects the way of its binding with DNA depending on the nature of the counterion in the solution.

 ДНК краунсодержащие производные феноксазона спектрофотометрия круговой дихроизм взаимодействие ионная сила среды DNA crown-containing derivatives of phenoxasone spectrophotometry spectropolarimetry interaction ionic strength of the medium

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