<!DOCTYPE article
PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.4 20190208//EN"
       "JATS-journalpublishing1.dtd">
<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" article-type="research-article" dtd-version="1.4" xml:lang="en">
 <front>
  <journal-meta>
   <journal-id journal-id-type="publisher-id">Russian Journal of Biological Physics and Chemisrty</journal-id>
   <journal-title-group>
    <journal-title xml:lang="en">Russian Journal of Biological Physics and Chemisrty</journal-title>
    <trans-title-group xml:lang="ru">
     <trans-title>АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ</trans-title>
    </trans-title-group>
   </journal-title-group>
   <issn publication-format="print">2499-9962</issn>
  </journal-meta>
  <article-meta>
   <article-id pub-id-type="publisher-id">54188</article-id>
   <article-categories>
    <subj-group subj-group-type="toc-heading" xml:lang="ru">
     <subject>МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ</subject>
    </subj-group>
    <subj-group subj-group-type="toc-heading" xml:lang="en">
     <subject>MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES</subject>
    </subj-group>
    <subj-group>
     <subject>МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ</subject>
    </subj-group>
   </article-categories>
   <title-group>
    <article-title xml:lang="en">DNA interaction with Co(II) compound containing 1,10-phenanthroline</article-title>
    <trans-title-group xml:lang="ru">
     <trans-title>Взаимодействие молекулы ДНК с соединением CO(II), содержащим 1,10-фенантролин</trans-title>
    </trans-title-group>
   </title-group>
   <contrib-group content-type="authors">
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Павленова</surname>
       <given-names>К Н</given-names>
      </name>
      <name xml:lang="en">
       <surname>Pavlenova</surname>
       <given-names>K N</given-names>
      </name>
     </name-alternatives>
     <email>pavlenova.kristina@gmail.com</email>
     <xref ref-type="aff" rid="aff-1"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Демидов</surname>
       <given-names>В Н</given-names>
      </name>
      <name xml:lang="en">
       <surname>Demidov</surname>
       <given-names>V N</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-2"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Касьяненко</surname>
       <given-names>Н А</given-names>
      </name>
      <name xml:lang="en">
       <surname>Kasyanenko</surname>
       <given-names>N A</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-3"/>
    </contrib>
   </contrib-group>
   <aff-alternatives id="aff-1">
    <aff>
     <institution xml:lang="ru">Санкт-Петербургский государственный университет</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Saint-Petersburg State University</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-2">
    <aff>
     <institution xml:lang="ru">Санкт-Петербургский государственный университет</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Saint-Petersburg State University</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-3">
    <aff>
     <institution xml:lang="ru">Санкт-Петербургский государственный университет</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Saint-Petersburg State University</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <pub-date publication-format="print" date-type="pub" iso-8601-date="2017-06-25T20:22:29+03:00">
    <day>25</day>
    <month>06</month>
    <year>2017</year>
   </pub-date>
   <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2017-06-25T20:22:29+03:00">
    <day>25</day>
    <month>06</month>
    <year>2017</year>
   </pub-date>
   <volume>2</volume>
   <issue>1</issue>
   <fpage>343</fpage>
   <lpage>347</lpage>
   <history>
    <date date-type="received" iso-8601-date="2017-06-20T20:22:29+03:00">
     <day>20</day>
     <month>06</month>
     <year>2017</year>
    </date>
    <date date-type="accepted" iso-8601-date="2017-06-20T20:22:29+03:00">
     <day>20</day>
     <month>06</month>
     <year>2017</year>
    </date>
   </history>
   <self-uri xlink:href="https://rusjbpc.ru/en/nauka/article/54188/view">https://rusjbpc.ru/en/nauka/article/54188/view</self-uri>
   <abstract xml:lang="ru">
    <p>Рассмотрено взаимодействие молекулы ДНК с координационным соединением двухвалентного кобальта, содержащим фенантролиновые лиганды, в растворе 0,005 M NaCl. Спектральные исследования показали, что в условиях эксперимента происходит образование комплексов. При этом в связывании с ДНК принимают участие фенантролиновые лиганды, входящие в состав соединения. Гипохромный эффект в спектре поглощения, наблюдаемый при образовании таких комплексов, может указывать на формирование димеров фенантролина или даже стопочных структур при связывании. Такое связывание достигает насыщения при r &gt; 0,4 (r - отношение молярных концентраций С соединения и ДНК, для которой С выражена в молях пар оснований). Одновременно с этим, по-видимому, осуществляется интеркаляция фенантролинового лиганда в двойную спираль ДНК, как это следует из данных вискозиметрии - происходит возрастание приведенной вязкости растворов ДНК примерно на 20%. Такое связывание достигает насыщения при r &gt; 0,2, а при r &gt; 0,4 начинается образование иных комплексов, что, напротив, вызывает падение вязкости растворов ДНК. Это падение не связано с уменьшением жесткости макромолекулы в результате нарушения вторичной структуры ДНК при связывании, так как, напротив, наблюдается ее стабилизация в комплексе, как это следует из изучения плавления ДНК в указанных условиях. Мы можем полагать, что кобальт способен образовывать координационную связь с молекулой ДНК. При этом один из фенантролиновых лигандов, который в результате такого связывания получил большую подвижность (одна связь с кобальтом разрушилась в результате координации кобальта к основанию ДНК), и который имеет большое сродство к основаниям ДНК, будет стремиться интеркалировать в двойную спираль. Оставшийся фенантролиновый лиганд может вступать во взаимодействие с аналогичными фенантролиновыми лигандами другого связавшегося с ДНК кобальта или со свободным фенантролином, который также может присутствовать в растворе в результате трансформации соединения кобальта при связывании с ДНК. Все эти взаимодействия и могут оказать влияние на изменение измеряемых параметров.</p>
   </abstract>
   <trans-abstract xml:lang="en">
    <p>DNA interaction with a coordination Co(II) compound containing phenanthroline ligands in a 5mM NaCl solution is studied. Spectral studies have shown that complex forms under experimental conditions. The phenanthroline ligands of the compound take part in binding to DNA. The hypochromic effect in the absorption spectrum upon the formation of these complexes is observed. It may indicate the formation of phenanthroline dimers or even stack structures upon binding. Such binding reaches saturation at r &gt; 0.4 (r is the ratio of the molar concentrations C of the compound and DNA (in moles of base pairs for DNA)). At the same time, it seems that the phenanthroline ligand is intercalated into the DNA double helix, as follows from the viscosimetry data - the viscosity of the DNA solutions increases by approximately 20%. This binding reaches saturation at r &gt; 0.2, and at r &gt; 0.4 the formation of another complexes begins, which causes a decrease of the DNA solutions viscosity. This decrease is not associated with a decrease of the DNA stiffness as a result of the breakdown of the secondary structure of DNA upon binding, because its stabilization in the complex is observed, as follows from the study of DNA melting. We can assume that cobalt is able to form a coordination bond with the DNA molecule. In this case, one of the phenanthroline ligands, which received a great mobility upon binding (one bond with cobalt was destroyed as a result of coordination binding of cobalt to the DNA base), and which has a great affinity for the DNA bases, will tend to intercalate into a double helix. The remaining phenanthroline ligand can interact with similar phenanthroline ligands of another cobalt bound to DNA or with free phenanthroline, which can be in solution because of transformation of the cobalt compound upon binding to DNA. All these interactions can influence the change of the measured parameters.</p>
   </trans-abstract>
   <kwd-group xml:lang="ru">
    <kwd>ДНК</kwd>
    <kwd>соединение кобальта с фенантролином</kwd>
    <kwd>образование комплекса</kwd>
    <kwd>интеркаляция</kwd>
   </kwd-group>
   <kwd-group xml:lang="en">
    <kwd>DNA</kwd>
    <kwd>Co(II) compound</kwd>
    <kwd>phenenthroline</kwd>
    <kwd>complexformation</kwd>
    <kwd>intercalation</kwd>
   </kwd-group>
  </article-meta>
 </front>
 <body>
  <p></p>
 </body>
 <back>
  <ref-list>
   <ref id="B1">
    <label>1.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Rosenberg B., VanCamp L., Trosko J.E., Mansour V.H. Platinum compounds: a new class of potent antitumour agents. Nature, 1969, vol. 222, pp. 385-386.</mixed-citation>
     <mixed-citation xml:lang="en">Rosenberg B., VanCamp L., Trosko J.E., Mansour V.H. Platinum compounds: a new class of potent antitumour agents. Nature, 1969, vol. 222, pp. 385-386.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B2">
    <label>2.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Wheate N.J., Walker S., Craig G.E., Oun R. The status of platinum anticancer drugs in the clinic and in clinical trials. Dalton Trans., 2010, vol. 39, pp. 8113-8127.</mixed-citation>
     <mixed-citation xml:lang="en">Wheate N.J., Walker S., Craig G.E., Oun R. The status of platinum anticancer drugs in the clinic and in clinical trials. Dalton Trans., 2010, vol. 39, pp. 8113-8127.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B3">
    <label>3.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Pitie M., Croisy A., Carrez D., Boldron C., Meunier B. Cytostatic activity of 1,10-phenanthroline derivatives generated by the clip-phen strategy. ChemBiochem, 2005, vol. 6, pp. 686-691.</mixed-citation>
     <mixed-citation xml:lang="en">Pitie M., Croisy A., Carrez D., Boldron C., Meunier B. Cytostatic activity of 1,10-phenanthroline derivatives generated by the clip-phen strategy. ChemBiochem, 2005, vol. 6, pp. 686-691.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B4">
    <label>4.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Demidov V.N., Kas'yanenko N.A., Antonov V.S., Volkov I.L., Sokolov P.A., Pakhomova T.B., Simanova S.A. Reaction with DNA and pharmacologic activity of 1,10-phenanthroline and electron-rich 1,10-phenanthrocyanine complexes of d-elements. Russian J. General Chem., 2012, vol. 82, pp. 602-620.</mixed-citation>
     <mixed-citation xml:lang="en">Demidov V.N., Kas'yanenko N.A., Antonov V.S., Volkov I.L., Sokolov P.A., Pakhomova T.B., Simanova S.A. Reaction with DNA and pharmacologic activity of 1,10-phenanthroline and electron-rich 1,10-phenanthrocyanine complexes of d-elements. Russian J. General Chem., 2012, vol. 82, pp. 602-620.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B5">
    <label>5.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Coyle B., Kinsella P., McCann M., Devereux M., O'Connor R., Clynes M., Kavanagh K. Induction of apoptosis in yeast and mammalian cells by exposure to 1,10-phenanthroline metal complexes. Toxicol. In Vitro, 2004, vol. 18, pp. 63-70.</mixed-citation>
     <mixed-citation xml:lang="en">Coyle B., Kinsella P., McCann M., Devereux M., O'Connor R., Clynes M., Kavanagh K. Induction of apoptosis in yeast and mammalian cells by exposure to 1,10-phenanthroline metal complexes. Toxicol. In Vitro, 2004, vol. 18, pp. 63-70.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B6">
    <label>6.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Erkkila K.E., Odom D.T., Barton J.K. Recognition and reaction of metallointercalators with DNA. Chem. Rev., 1999, vol. 99, pp. 2777-2795.</mixed-citation>
     <mixed-citation xml:lang="en">Erkkila K.E., Odom D.T., Barton J.K. Recognition and reaction of metallointercalators with DNA. Chem. Rev., 1999, vol. 99, pp. 2777-2795.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B7">
    <label>7.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Brodie C.R., Collins J.G., Aldrich-Wright J.R. DNA binding and biological activity of some platinum(II) intercalating compounds containing methyl-substituted 1,10-phenanthrolines. Dalton Trans., 2004, vol. 8, vol. 1145-1152.</mixed-citation>
     <mixed-citation xml:lang="en">Brodie C.R., Collins J.G., Aldrich-Wright J.R. DNA binding and biological activity of some platinum(II) intercalating compounds containing methyl-substituted 1,10-phenanthrolines. Dalton Trans., 2004, vol. 8, vol. 1145-1152.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B8">
    <label>8.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Lu W., Vicic D.A., Barton J.K. Reductive and oxidative DNA damage by photoactive platinum(II) intercalators. Inorg. Chem., 2005, vol. 44, pp. 7970-7980.</mixed-citation>
     <mixed-citation xml:lang="en">Lu W., Vicic D.A., Barton J.K. Reductive and oxidative DNA damage by photoactive platinum(II) intercalators. Inorg. Chem., 2005, vol. 44, pp. 7970-7980.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B9">
    <label>9.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Osinsky S., Levitin I., Sigan A., Bubnovskaya L., Ganusevich I., Campanella L., Wardman P. Redox-active cobalt complexes as promising antitumor agents. Russian Chemical Bulletin, 2003, vol. 52, pp. 2636-2645.</mixed-citation>
     <mixed-citation xml:lang="en">Osinsky S., Levitin I., Sigan A., Bubnovskaya L., Ganusevich I., Campanella L., Wardman P. Redox-active cobalt complexes as promising antitumor agents. Russian Chemical Bulletin, 2003, vol. 52, pp. 2636-2645.</mixed-citation>
    </citation-alternatives>
   </ref>
   <ref id="B10">
    <label>10.</label>
    <citation-alternatives>
     <mixed-citation xml:lang="ru">Kasyanenko N., Qiushi Z., Bakulev V., Osolodkov M., Sokolov P., Demidov V DNA binding with acetate bis(1,10- phenanthroline)silver(I) monohydrate in a solution and metallization of formed structures. Polymers, 2017, vol. 9, iss. 6, pp. 211-230.</mixed-citation>
     <mixed-citation xml:lang="en">Kasyanenko N., Qiushi Z., Bakulev V., Osolodkov M., Sokolov P., Demidov V DNA binding with acetate bis(1,10- phenanthroline)silver(I) monohydrate in a solution and metallization of formed structures. Polymers, 2017, vol. 9, iss. 6, pp. 211-230.</mixed-citation>
    </citation-alternatives>
   </ref>
  </ref-list>
 </back>
</article>
