Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54192 МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ EPR-spectroscopy of biogenic crystalline iron oxides in biological tissues ЭПР-спектроскопия биогенных кристаллических оксидов железа в биологических тканях Юртаева С В Yurtaeva S V s.yurtaeva@kfti.knc.ru Ефимов В Н Efimov V N vefimov.51@mail.ru Сальников В В Salnikov V V salnikov_russ@yahoo.com Казанский физико-технический институт Казанского научного центра РАН ru Kazan Physical technical Institute of Russian Academy of Sciences ru Казанский федеральный университет ru Kazan Federal University ru Казанский институт биохимии и биофизики Казанского научного центра РАН ru Kazan Institute of Biochemistry and Biophysics of Russian Academy of Sciences ru 25 06 2017 25 06 2017 2 1 360 365 20 06 2017 20 06 2017 https://rusjbpc.ru/en/nauka/article/54192/view

Изучено поведение сигналов ЭМР в биологических тканях различного происхождения (ткани крыс с моделями различных патологий, ткани злокачественных опухолей, ткани нервного ганглия и ткани сердца виноградной улитки) в широком диапазоне температур. Проанализированы характеристики сигналов ЭМР в перечисленных тканях. Установлены общие характеристики сигналов ЭМР, которые в большинстве случаев свидетельствуют об образовании кристаллов магнетита в тканях: характерное немонотонное температурное поведение резонансного поля, ширины линии и интегральной интенсивности, наличие аксиальной и кубической компонент в анизотропии резонансного поля. Обнаружен Фазовый переход Вервея. При температурах выше температуры фазового перехода Вервея обнаруживается корреляция величин ширины линии и резонансного поля с параметром магнетокристаллической анизотропии магнетита. Обнаружены различные типы анизотропного поведения резонансного поля, характеризующие различную геометрию кристаллических включений. Описанные сигналы ЭМР в биологических тканях и крови могут быть использованы для детектирования аномального накопления железа в кристаллической форме.

The characteristics of EMR signals in different biological tissues (tissues of laboratory rats with biological models of different pathologies, cancer tumor tissues, rat tissues after effect of hypogravitation, nerve tissue of snail Helix Luccorum ) were investigated. The general characteristics of EMR signals were determined: characteristic non-monotonic temperature behavior of resonance field (Hres), linewidth (ΔH) and integral intensity, the existence of axial and cubic components in Hres anisotropy. Vervey phase transition, character for magnetite was detected. The characteristics indicate that there are magnetite crystals in tissues. At the temperatures higher the Verwey transition the correlation of resonance field and linewidth with magnetic crystalline anisotropy parameter K1 was detected. The different types of anisotropic behavior of signals were detected. It was determined, that they characterize the different types of geometry of crystalline inclusions. Magnetic resonance investigation of EMR signals in biological tissues and blood is suggested to use as the method of detection of anomalous accumulation of tissue iron in the forms of iron oxide nanocrystals (magnetite and ferritin).

 биоминерализация железа ЭПР-спектроскопия сигналы ЭМР ферритин ферригидрит магнетит iron biomineralization EPR-Spectroscopy EMR-signals ferritin ferrihydrite magnetite

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