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 <front>
  <journal-meta>
   <journal-id journal-id-type="publisher-id">Russian Journal of Biological Physics and Chemisrty</journal-id>
   <journal-title-group>
    <journal-title xml:lang="en">Russian Journal of Biological Physics and Chemisrty</journal-title>
    <trans-title-group xml:lang="ru">
     <trans-title>АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ</trans-title>
    </trans-title-group>
   </journal-title-group>
   <issn publication-format="print">2499-9962</issn>
  </journal-meta>
  <article-meta>
   <article-id pub-id-type="publisher-id">54195</article-id>
   <article-categories>
    <subj-group subj-group-type="toc-heading" xml:lang="ru">
     <subject>НАНОБИОФИЗИКА</subject>
    </subj-group>
    <subj-group subj-group-type="toc-heading" xml:lang="en">
     <subject>NANOBIOPHYSICS</subject>
    </subj-group>
    <subj-group>
     <subject>НАНОБИОФИЗИКА</subject>
    </subj-group>
   </article-categories>
   <title-group>
    <article-title xml:lang="en">Preparation of magnetically controlled human cells by labeling with Fe3O4 magnetic nanoparti-cles at short time of exposition</article-title>
    <trans-title-group xml:lang="ru">
     <trans-title>Получение магнитоуправляемых клеток человека путем маркировки магнитными наноча-стицами Fe3O4 при малом времени экспозиции</trans-title>
    </trans-title-group>
   </title-group>
   <contrib-group content-type="authors">
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Солопов</surname>
       <given-names>М В</given-names>
      </name>
      <name xml:lang="en">
       <surname>Solopov</surname>
       <given-names>M V</given-names>
      </name>
     </name-alternatives>
     <email>mxsolopov@yandex.ru</email>
     <xref ref-type="aff" rid="aff-1"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Турчин</surname>
       <given-names>Виктор Васильевич</given-names>
      </name>
      <name xml:lang="en">
       <surname>Turchyn</surname>
       <given-names>V V</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-2"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Лёгенький</surname>
       <given-names>Ю А</given-names>
      </name>
      <name xml:lang="en">
       <surname>Legenkiy</surname>
       <given-names>Yu A</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-3"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Попандопуло</surname>
       <given-names>Андрей Геннадиевич</given-names>
      </name>
      <name xml:lang="en">
       <surname>Popandopulo</surname>
       <given-names>A G</given-names>
      </name>
     </name-alternatives>
     <bio xml:lang="ru">
      <p>доктор медицинских наук;</p>
     </bio>
     <bio xml:lang="en">
      <p>doctor of medical sciences;</p>
     </bio>
     <xref ref-type="aff" rid="aff-4"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Беспалова</surname>
       <given-names>С В</given-names>
      </name>
      <name xml:lang="en">
       <surname>Bespalova</surname>
       <given-names>S V</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-5"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Глазунова</surname>
       <given-names>В А</given-names>
      </name>
      <name xml:lang="en">
       <surname>Glazunova</surname>
       <given-names>V A</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-6"/>
    </contrib>
   </contrib-group>
   <aff-alternatives id="aff-1">
    <aff>
     <institution xml:lang="ru">Донецкий национальный университет; Институт неотложной и восстановительной хирургии им. В.К. Гусака</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Donetsk National University; V.K. Gusak Institute of Urgent and Recovery Surgery</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-2">
    <aff>
     <institution xml:lang="ru">Институт неотложной и восстановительной хирургии им. В.К. Гусака</institution>
     <city>Донецк</city>
     <country>Россия</country>
    </aff>
    <aff>
     <institution xml:lang="en">V.K. Gusak Institute of Urgent and Recovery Surgery</institution>
     <city>Donetsk</city>
     <country>Russian Federation</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-3">
    <aff>
     <institution xml:lang="ru">Донецкий национальный университет</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Donetsk National University</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-4">
    <aff>
     <institution xml:lang="ru">Институт неотложной и восстановительной хирургии им. В.К. Гусака</institution>
     <city>Донецк</city>
     <country>Россия</country>
    </aff>
    <aff>
     <institution xml:lang="en">V.K. Gusak Institute of Urgent and Recovery Surgery</institution>
     <city>Donetsk</city>
     <country>Russian Federation</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-5">
    <aff>
     <institution xml:lang="ru">Донецкий национальный университет</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Donetsk National University</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-6">
    <aff>
     <institution xml:lang="ru">Донецкий физико-технический институт им. А.А. Галкина</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">A.A. Galkin Donetsk Physicotechnical Institute</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <pub-date publication-format="print" date-type="pub" iso-8601-date="2017-06-25T20:22:29+03:00">
    <day>25</day>
    <month>06</month>
    <year>2017</year>
   </pub-date>
   <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2017-06-25T20:22:29+03:00">
    <day>25</day>
    <month>06</month>
    <year>2017</year>
   </pub-date>
   <volume>2</volume>
   <issue>1</issue>
   <fpage>372</fpage>
   <lpage>376</lpage>
   <history>
    <date date-type="received" iso-8601-date="2017-06-20T20:22:29+03:00">
     <day>20</day>
     <month>06</month>
     <year>2017</year>
    </date>
    <date date-type="accepted" iso-8601-date="2017-06-20T20:22:29+03:00">
     <day>20</day>
     <month>06</month>
     <year>2017</year>
    </date>
   </history>
   <self-uri xlink:href="https://rusjbpc.ru/en/nauka/article/54195/view">https://rusjbpc.ru/en/nauka/article/54195/view</self-uri>
   <abstract xml:lang="ru">
    <p>В связи с активным развитием клеточной трансплантологии, как отрасли регенеративной медицины, разработка методик точного нацеливания клеток in vivo представляется актуальной. Всё большую популярность приобретает использование магнитных наночастиц в качестве маркеров, как инструмента для получения управляемых клеточных препаратов. В результате магнитной маркировки клетка приобретает возможность быть управляемой с помощью магнитного поля. В представленной работе исследовались магнитные свойства фетальных фибробластов человека (ФФЧ), полученных после инкубации с наночастицами Fe3O4 при малом времени экспозиции. Были определены такие показатели модифицированных клеток, как жизнеспособность, внутриклеточное содержание железа, а также магнитная восприимчивость (χ). На основе расчетных экспериментальных данных была построена компьютерная модель захвата клеток в условиях кровеносного русла в присутствии внешнего магнитного поля. Результаты моделирования указывают на то, что эффективность захвата маркированных клеток, полученных по описанной методике, линейно зависит от значения χ и может составлять около 60 %.</p>
   </abstract>
   <trans-abstract xml:lang="en">
    <p>Due to the active progress of cell transplantology as branch of regenerative medicine, development of techniques for precise cell targeting in vivo has a great relevance. Application of magnetic nanoparticles as cell markers becoming a rather popular tool for obtaining of controlled cell products. As a result of magnetic labeling, the marked cell acquires the possibility of being controlled by means of a magnetic field. In the presented work the magnetic properties of human fetal fibroblasts (HFF) obtained after incubation with Fe3O4 nanoparticles at a short exposure time were investigated. Such characteristics of the modified cells as viability, intracellular iron content and magnetic susceptibility (χ) were defined. A computer model of cell capture in the conditions of the blood channel in the presence of an external magnetic field based on calculated experimental data was constructed. The results of the simulation indicate that the capture efficiency of the labeled cells obtained by the described procedure is linearly dependent on the value of χ and can reach about 60 %.</p>
   </trans-abstract>
   <kwd-group xml:lang="ru">
    <kwd>магнитные наночастицы</kwd>
    <kwd>магнитное поле</kwd>
    <kwd>магнитная восприимчивость</kwd>
    <kwd>фетальные фибробласты человека</kwd>
   </kwd-group>
   <kwd-group xml:lang="en">
    <kwd>magnetic nanoparticles</kwd>
    <kwd>magnetic field</kwd>
    <kwd>magnetic susceptibility</kwd>
    <kwd>human fetal fibroblasts</kwd>
   </kwd-group>
  </article-meta>
 </front>
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