Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54213 БИООРГАНИЧЕСКАЯ, БИОФИЗИЧЕСКАЯ И МЕДИЦИНСКАЯ ХИМИЯ BIOORGANIC, BIOPHYSICAL AND MEDICINAL CHEMISTRY БИООРГАНИЧЕСКАЯ, БИОФИЗИЧЕСКАЯ И МЕДИЦИНСКАЯ ХИМИЯ Delayed fluorescence of sensitizer in biological tissues with low oxygen tension Длительная люминесценция сенсибилизатора в биологических тканях в условиях дефицита кислорода Ишемгулов А Т Ishemgulov A T azamat.ischemgulov@yandex.ru Летута С Н Letuta S N letuta@com.osu.ru Пашкевич С Н Pashkevich S N Оренбургский государственный университет ru Orenburg State University ru Оренбургский государственный университет ru Orenburg State University ru Оренбургский государственный университет ru Orenburg State University ru 25 06 2017 25 06 2017 2 1 442 446 20 06 2017 20 06 2017 https://rusjbpc.ru/en/nauka/article/54213/view

Исследована кинетика затухания замедленной люминесценции ксантеновых зондов при варьировании уровня кислорода в биологических тканях. Показано, что значительная доля замедленной флуоресценции зонда образцов, находящихся на открытом воздухе, генерируется за счёт аннигиляции триплет-возбуждённого сенсибилизатора и синглетного кислорода (СТА-ЗФ). При снижении концентрации кислорода в тканях, в том числе и в результате фотодинамического эффекта, наблюдается тушение СТА-ЗФ. Показывается, что чувствительность СТА-ЗФ к содержанию синглетного кислорода в ткани выше, чем других видов замедленной люминесценции сенсибилизатора. Предлагается по величине интенсивности СТА-ЗФ можно отслеживать динамику кислорода в тканях в режиме реального времени. Преимущества данного подхода перед альтернативными методиками мониторинга свободного кислорода в тканях также обсуждаются.

Delayed luminescence kinetics of xanthene dyes was investigated for biological tissues with various oxygen tensions. It was shown that a large part of sensitizer delayed fluorescence is generated through annihilation of triplet-excited sensitizer and singlet oxygen (STA-DF) when samples are in open air. If tissue oxygen tension decreases including as a result of the photodynamic effect, quenching of STA-DF is observed. It is shown that the STA-DF has higher sensitivity to singlet oxygen amount in the tissue than other types of sensitizer delayed luminescence. It is proposed to monitor the oxygen dynamics in the tissue by the magnitude of the STA-DF intensity in real time. Some advantages of this approach over alternative fluorescent techniques of tissue oxygen monitoring are discussed.

 синглетный кислород оксигенация биологических тканей синглет-триплетная аннигиляция замедленная флуоресценция singlet oxygen biological tissue oxygenation singlet-triplet annihilation delayed fluorescence

Ormond A.B., Freeman H.S. Dye Sensitizers for Photodynamic Therapy. Materials, 2013, vol. 6, pp. 817-840. Ormond A.B., Freeman H.S. Dye Sensitizers for Photodynamic Therapy. Materials, 2013, vol. 6, pp. 817-840. Hilderbrand S.A. Labels and Probes for Live Cell Imaging: Overview and Selection Guide Live Cell. Imaging Methods in Molecular Biology, 2010, vol. 591, pp. 17-45. Hilderbrand S.A. Labels and Probes for Live Cell Imaging: Overview and Selection Guide Live Cell. Imaging Methods in Molecular Biology, 2010, vol. 591, pp. 17-45. Nyokong T. Photosensitizers in Medicine, Environment, and Security. Springer Science + Business Media B.V., 2012, ISBN: 978-90-481-3870-8 (Print), 978-90-481-3872-2 (Online). Nyokong T. Photosensitizers in Medicine, Environment, and Security. Springer Science + Business Media B.V., 2012, ISBN: 978-90-481-3870-8 (Print), 978-90-481-3872-2 (Online). Swartz H.M. Measuring real levels of oxygen in vivo: opportunities and challenges. Biochem. Soc. Trans, 2002, vol. 30, pp. 248-252. Swartz H.M. Measuring real levels of oxygen in vivo: opportunities and challenges. Biochem. Soc. Trans, 2002, vol. 30, pp. 248-252. Dmitriev R.I., Papkovsky D.B. Optical probes and techniques for O2 measurement in live cells and tissue. Cell. Mol. Life Sci, 2012, vol. 69, pp. 2025-2039. Dmitriev R.I., Papkovsky D.B. Optical probes and techniques for O2 measurement in live cells and tissue. Cell. Mol. Life Sci, 2012, vol. 69, pp. 2025-2039. Baier J. [et al.] Time-Resolved Investigations of Singlet Oxygen Luminescence in Water, in Phosphatidylcholine, and in Aqueous Suspensions of Phosphatidylcholine or HT29 Cells. J. Phys. Chem. B, 2005, vol. 109, no. 7, pp. 3041-3046. Baier J. [et al.] Time-Resolved Investigations of Singlet Oxygen Luminescence in Water, in Phosphatidylcholine, and in Aqueous Suspensions of Phosphatidylcholine or HT29 Cells. J. Phys. Chem. B, 2005, vol. 109, no. 7, pp. 3041-3046. Skovsen E., Snyder J.W., Lambert J.D., Ogilby P.R. Lifetime and diffusion of singlet oxygen in a cell. J. Phys. Chem B, 2005, vol. 109, no. 18, pp. 8570-8573. Skovsen E., Snyder J.W., Lambert J.D., Ogilby P.R. Lifetime and diffusion of singlet oxygen in a cell. J. Phys. Chem B, 2005, vol. 109, no. 18, pp. 8570-8573. Dědic R., Svoboda A., Psencik J., Hala J. Phosphorescence of singlet oxygen and meso-tetra (4-sulfonatophenyl)porphin: time and spectral resolved study. J. Mol. Struct, 2003, vol. 651, pp. 301-304. Dědic R., Svoboda A., Psencik J., Hala J. Phosphorescence of singlet oxygen and meso-tetra (4-sulfonatophenyl)porphin: time and spectral resolved study. J. Mol. Struct, 2003, vol. 651, pp. 301-304. Piffaretti F. [et al.] Real-time, in vivo measurement of tissular pO2 through the delayed fluorescence of endogenous protoporphyrin IX during photodynamic therapy. J. Biomed. Opt, 2012, vol. 17, no. 11, pp. 115007. Piffaretti F. [et al.] Real-time, in vivo measurement of tissular pO2 through the delayed fluorescence of endogenous protoporphyrin IX during photodynamic therapy. J. Biomed. Opt, 2012, vol. 17, no. 11, pp. 115007. Mik E.G. [et al.] In vivo mitochondrial oxygen tension measured by a delayed fluorescence lifetime technique. Biophys. J, 2008, vol. 95, pp. 3977-3990. Mik E.G. [et al.] In vivo mitochondrial oxygen tension measured by a delayed fluorescence lifetime technique. Biophys. J, 2008, vol. 95, pp. 3977-3990. Wilson D.F. Oxygen dependent quenching of phosphorescence: a perspective. Adv. Exp. Med. Biol, 1992, vol. 317, pp. 195-201. Wilson D.F. Oxygen dependent quenching of phosphorescence: a perspective. Adv. Exp. Med. Biol, 1992, vol. 317, pp. 195-201. Lin H. Shen Y., Chen D., Lin L. Feasibility Study on Quantitative Measurements of Singlet Oxygen Generation Using Singlet Oxygen Sensor Green. J. Fluoresc, 2013, vol. 23, pp. 41-47. Lin H. Shen Y., Chen D., Lin L. Feasibility Study on Quantitative Measurements of Singlet Oxygen Generation Using Singlet Oxygen Sensor Green. J. Fluoresc, 2013, vol. 23, pp. 41-47. Lee S. [et al.] Pulsed diode laser-based monitor for singlet molecular oxygen. J. Biomed. Opt, 2008, vol. 13, no. 3, pp. 034010. Lee S. [et al.] Pulsed diode laser-based monitor for singlet molecular oxygen. J. Biomed. Opt, 2008, vol. 13, no. 3, pp. 034010. Scholz M., Biehl A., Dedic R., Hala J. Singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopic approach. Photochem. Photobiol. Sci., 2015, vol. 14, no. 4, pp. 700-713. Scholz M., Biehl A., Dedic R., Hala J. Singlet-oxygen-sensitized delayed fluorescence in mammalian cells: a time-resolved microscopic approach. Photochem. Photobiol. Sci., 2015, vol. 14, no. 4, pp. 700-713. Letuta S. N. Kuvandykova A.F., Pashkevich S.N., Saletskii A.M. Features of the delayed fluorescence kinetics of exogenous fluorophores in biological tissues. Russ. J. Phys. Chem. A, 2013, vol. 87, pp. 1582-1587. Letuta S. N. Kuvandykova A.F., Pashkevich S.N., Saletskii A.M. Features of the delayed fluorescence kinetics of exogenous fluorophores in biological tissues. Russ. J. Phys. Chem. A, 2013, vol. 87, pp. 1582-1587. Letuta S.N. [et al.] Delayed luminescence of erythrosine in biological tissue and photodynamic therapy dosimetry. J. Photochem. Photobiol. B, 2016, vol. 163, pp. 232-236. Letuta S.N. [et al.] Delayed luminescence of erythrosine in biological tissue and photodynamic therapy dosimetry. J. Photochem. Photobiol. B, 2016, vol. 163, pp. 232-236. Celli J.P. [et al.] Imaging and photodynamic therapy: mechanisms, monitoring, and optimization. Chem. Rev, 2010, vol. 110, pp. 2795-2838. Celli J.P. [et al.] Imaging and photodynamic therapy: mechanisms, monitoring, and optimization. Chem. Rev, 2010, vol. 110, pp. 2795-2838. Woodhams J.H., MacRobert A.J., Bown S.G. The role of oxygen monitoring during photodynamic therapy and its potential for treatment dosimetry. Photochem. Photobiol. Sci, 2007, vol. 6, pp. 1246-1256. Woodhams J.H., MacRobert A.J., Bown S.G. The role of oxygen monitoring during photodynamic therapy and its potential for treatment dosimetry. Photochem. Photobiol. Sci, 2007, vol. 6, pp. 1246-1256. Moiseeva E.V. Original Approaches to Test Anti-breast Cancer Drugs in a Novel Set of Mouse Models. Proefschrift Universiteit Utrecht, 2005, 191 p. Moiseeva E.V. Original Approaches to Test Anti-breast Cancer Drugs in a Novel Set of Mouse Models. Proefschrift Universiteit Utrecht, 2005, 191 p.