The re-understand of the probabilistic possibilities of creating pre-biotic and biotic processes, resulting in initiation, further development and adaptation to environment of predecessors of living subjects are presented at this work. For these purposes the certain data were obtained, when studying the actions of biologically active substance (BAS) with engagement of modern experimental models and bio-objects. The experimental objects used consistently as of complicating of their structural properties and respectively of emergence of functional properties. The liposomes, which are formed, from artificial individual phospholipid, and from mixture of nature phospholipids, were used as the experimental objects for structural studies of lipid-lipid interactions. To identify of structural rearrangements, which involved of the protein-lipid interactions, the erythrocyte ghost and the insulated erythrocytes were used. The investigations of BAS influences on cells functioning were provided with study of calcium signalizations ways of the transformed with uncontrolled growth cells of ascetic Ehrlich carcinoma, and of the normal cells - thymocytes and lymphocytes. For inspection of actions of BAS as the modifiers of structures and functions of model and bio-objects, we used hydrophilic substances: the plant growth regulator melafen, the antioxidant phenoksan, and the hydrophobe antioxidants - IHFANs. So has managed to trace influence BAS, as probable factors of natural selection of different nature on increasingly becoming complicated on its organization the objects, simulating the development stages at an earlier stages of life-history. BAS may be regarded, as the factors of evolution. BAS may be factors for elimination of instable structures. And BAS may be also as strong factors for emergence of new subjects or updating of existence subjects due to BAS incorporating to its structures. The .obtained data could help also to performance analysis of predecessors of modern cellular structures.