Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54257 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ ON THE NATURE OF THE EMITTER IN LUCIFERIN-LUCIFERASE SYSTEM OF FIREFLIES О ПРИРОДЕ ЭМИТТЕРА В ЛЮЦИФЕРИН-ЛЮЦИФЕРАЗНОЙ СИСТЕМЕ СВЕТЛЯКОВ Угарова Н Н Ugarova N N nugarova@gmail.com Ломакина Г Ю Lomakina G Yu Московский государственный университет имени М.В. Ломоносова ru Lomonosov Moscow State University ru Московский государственный университет имени М.В. Ломоносова ru Lomonosov Moscow State University ru 25 03 2018 25 03 2018 3 1 133 138 20 03 2018 20 03 2018 https://rusjbpc.ru/en/nauka/article/54257/view

Отличительной особенностью биолюминесценции люцифераз светляков являются сложные спектральные изменения в форме и максимумах биолюминесценции при варьировании рН, температуры, структуры фермента. Анализ литературных данных и собственных результатов авторов о природе эмиттера в люциферин-люциферазной системе светляков приводит к заключению, что кето-энольная таутомерия молекулы оксилюциферина наиболее достоверно объясняет наблюдаемые сложные спектральные изменения. Каждая молекула люциферазы может содержать лишь одну молекулу эмиттера в момент биолюминесценции, поэтому можно рассматривать эмиттер как внутримолекулярную метку, характеризующую свойства ее микроокружения в активном центре фермента. Суперпозиция двух или трех форм эмиттера, фиксируемых в спектрах биолюминесенции, указывает на то, что в реакционной среде сосуществуют различные конформационные формы фермента, находящиеся в динамическом равновесии, каждая из которых генерирует одну из форм эмиттера.

The distinctive features of firefly luciferase bioluminescence are the complex spectral changes of the form and symbol 108 \f "Symbol" \s 12lмакс of bioluminescence under variation of pH, temperature, and enzyme structure. Analysis of the literature data and the own results of the authors on the nature of the emitter in luciferin-luciferase system leads to the conclusion that the keto-enol tautomerism of oxyluciferin molecule the most authentically explains observable complex spectral changes. Each molecule of luciferase can contain only one molecule of the emitter at the moment of bioluminescence. Hence, the emitter can be considered as the intramolecular label, characterizing the properties of its microenvironment in the active center of the enzyme. Superposition of two or three forms of the emitter recorded in bioluminescence spectra indicates that various conformational forms of the enzyme co-exist in the reaction medium, that are in a dynamic equilibrium, each of which generates one of the emitter forms.

 биолюминесценция люцифераза светляков кето-енольная таутомерия оксилюциферин bioluminescence firefly luciferase keno-enol tautomerism oxyluciferin

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