Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54265 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ BIOMATERIALS AND TO STUDY THEIR DISTRIBUTION IN THE BODY OF ANIMALS AFTER INTRAVENOUS INJECTION ИСПОЛЬЗОВАНИЕ ЭПР-СПЕКТРОМЕТРИИ ДЛЯ ВЫЯВЛЕНИЯ НАНОАЛМАЗОВ В БИОМАТЕРИАЛАХ И ИЗУЧЕНИЯ ИХ РАСПРЕДЕЛЕНИЯ В ОРГАНИЗМЕ ЖИВОТНЫХ ПОСЛЕ ВНУТРИВЕННОГО ВВЕДЕНИЯ Инжеваткин Е В Inzhevatkin E V inscience@mail.ru Барон А В Baron A V Максимов Н Г Maksimov N G Волкова М Б Volkova M B Пузырь А. П. Puzyr A. P. Ронжин Н О Ronzhin N O Бондарь В С Bondar V S bondvs@mail.ru Институт биофизики ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук”; Международный научный центр исследований экстремальных состояний организма ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук” ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”; International Scientific Center for Studies of Extreme States of Organism, Federal Research Center “Krasnoyarsk Science Center SB RAS” ru Институт биофизики ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук”; Сибирский федеральный университет ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”; Siberian Federal University ru Институт биофизики ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук”; Институт химии и химической технологии ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук” ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”; Institute of Chemistry and Chemical Technology, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS” ru Институт биофизики ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук”; Сибирский федеральный университет ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS”; Siberian Federal University ru ФИЦ КНЦ СО РАН Красноярск Россия FRC KSC SB RAS Krasnoyarsk Russian Federation ФИЦ КНЦ СО РАН Красноярск Россия FRC KSC SB RAS Krasnoyarsk Russian Federation Институт биофизики ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук” ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS” ru Институт биофизики ФИЦ “Красноярский научный центр Сибирского отделения Российской Академии наук” ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS” ru 25 03 2018 25 03 2018 3 1 183 188 20 03 2018 20 03 2018 https://rusjbpc.ru/en/nauka/article/54265/view

В модельных экспериментах in vitro установлена применимость метода ЭПР-спектрометрии для выявления модифицированных наноалмазов (МНА) взрывного синтеза в образцах биоматериалов (кровь и гомогенаты органов мышей). В содержащих МНА биопробах регистрируется характерный ЭПР сигнал (g = 2,003, ΔH ≈ 10 Гс), интенсивность которого линейно возрастает при концентрации наночастиц в диапазоне 1,6-200 мкг МНА на 1 мл образца. В экспериментах in vivo методом ЭПР установлено, что через 2.5 часа после внутривенной инъекции мышам МНА аккумулируются преимущественно в легких и печени животных - около 25 % и 20 %, соответственно, от дозы введенных наночастиц. В сердце и почках животных было обнаружено значительно меньшее (на 1-1,5 порядка) количество МНА. В крови, селезенке, головном мозге и мышцах бедра мышей наличие МНА не было выявлено в пределах чувствительности метода ЭПР. Через 10 суток после инъекции регистрируется перераспределение МНА в организме животных: количество наночастиц в легких уменьшается в 3,5 раза и увеличивается в печени практически в 3 раза, наблюдается накопление МНА в селезенке. В это время не наблюдалось изменений в содержании МНА в сердце и почках мышей. В целом, полученные данные открывают перспективы использования ЭПР-спектрометрии для изучения динамики межорганного распределения, накопления и элиминации МНА при их внутривенном введении в организм экспериментальных животных.

In the model experiments in vitro , the applicability of EPR spectrometry for the detection of the modified detonation nanodiamonds (MNDs) in biomaterial samples (blood and homogenates of mouse organs) was established. The intensity of a characteristic EPR signal (g = 2.003, ΔH ≈ 10 G) registered in the samples increases linearly at MNDs concentrations in the range of 1.6-200 μg/ml. It was established in experiments in vivo that 2.5 hours after an intravenous injection to mice MNDs were mainly accumulated in the lungs and liver of animals - about 25 % and 20 % of the injected dose, respectively. A much smaller amount of MNDs (by 1-1.5 orders of magnitude) was found in the heart and kidneys. The presence of MNDs in the blood, spleen, brain and hip muscles was not detected within the sensitivity of the EPR method. Ten days after injection, the redistribution of MNDs was recorded: the amount of nanoparticles in the lungs decreases 3.5 fold and increases practically 3 fold in the liver. There was also registered the accumulation of MNDs in the spleen. At the same time, no change in the content of MNDs in the heart and kidneys was observed. In general, the obtained results open up the prospects for using EPR spectrometry to study the dynamics of inter-organs distribution, accumulation and elimination of MNDs after intravenous injection to the body of experimental animals.

 наноалмазы ЭПР-спектрометрия биологические образцы кровь гомогенаты органов динамика распределения nanodiamonds EPR spectrometry biological samples blood organs homogenates distribution dynamics

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