Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54266 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS ОКИСЛИТЕЛЬНАЯ МОДИФИКАЦИЯ ЭРИТРОЦИТОВ УМЕРЕННЫМИ ДОЗАМИ НИТРИТОМ НАТРИЯ В ОПЫТАХ in vitro Гусейнова С Я Huseynova S Ya Гусейнов Т М Huseynov T M thuseynov@physics.ab.az Дадашов М З Dadashov M Z Институт Биофизики, НАН Азербайджана ru Institute of Biophysics, NAS of Azerbaijan ru Институт Биофизики, НАН Азербайджана ru Institute of Biophysics, NAS of Azerbaijan ru Институт Биофизики, НАН Азербайджана ru Institute of Biophysics, NAS of Azerbaijan ru 25 03 2018 25 03 2018 3 1 189 195 20 03 2018 20 03 2018 https://rusjbpc.ru/en/nauka/article/54266/view

В работе изучена влияния умеренно-хронических доз нитрита натрия на окислительной модификации Hb и эритроцитов, изменения активности каталазы, глутатионпероксидазы (ГП) и интенсивность перекисное окисления липидов (ПОЛ). Анализ спектральных линий раман спектроскопии показывает, что способность Hb связывать кислород I1580/I1548 в суспензии эритроцитов, обработанных NaNO2 существенно выше, чем в контроле и этот эффект прямо зависит от концентрации NaNO2 и значителен уже при минимальных концентрациях NaNO2 (0,07 мМ), а относительное способность Hb отдавать лиганды I1375/I1580 также зависим от дозы, но в противоположную сторону. Проявления симметричных и асимметричных колебаний пиррольных колец Hb (I1375/I1172) с увеличением дозы нитрита повышается на ~ 14 %, что указывает о наличие конформационных изменений пиррольных колец, связанных с окислительной модификацией Hb. Накопление metHb также зависим от от дозы и достигает своего максимума на 30-40 минутах, это сопровождается интенсификацией процессов ПОЛ и увеличением доли мембрансвязаного Hb. Характерно, что развитие окислительного процесса сопровождается уменьшением активности каталазы, а активность ГП изменяется неоднозначно и при повышенных содержаниях NaNO2 несколько растет. Констатируя указанных фактов можно сказать, что уже относительно малые концентрации нитрита натрия могут влиять на конформацию Hb, приводящую к изменению его сродства к О2. Это стимулирует окислительную модификацию Hb, что отражается в росте накопления metHb и потери активности каталазы и ГП, а при больших концентрациях нитрита и к росту активности ГП.

The work explored the influence of moderately chronic doses of NaNO2 on the oxidative modification of Hb and RBC, changes the activity of catalase, glutathione peroxidase (GP) and the intensity of peroxide oxidation of lipids (LPO). Analysis of the spectral lines of Raman Spectroscopy shows that the ability of the Hb to bind O2 I1580/I1548 in a suspension of erythrocytes treated with NaNO2 is significantly higher than control and this effect directly depends on the concentration of NaNO2 and significant even at the minimum concentrations of NaNO2 (0,07 mm), and relative ability of Hb to give ligands I1375/I1580 is also dose dependent but in the opposite direction. Manifestations of symmetrical and asymmetrical vibrations of the pyrrole rings of Hb (I1375/I1172) increase with an increasing nitrite dose by ~ 14 %, which indicates the presence of conformational changes in the pyrrole rings associated with the oxidative modification of Hb. MetHb accumulation also dose-depend and reaches its maximum at 30-40 minutes, this is accompanied by the intensification of processes of LPO and the increasing share of membrane bound Hb. It is characteristic that the development of oxidative process is accompanied by a decrease in catalase activity and the activity of GP changes ambiguously and multiple grows with increased NaNO2 contents. Noting these facts, we can say that the already relatively small concentrations of nitrite of sodium can affect the conformation of Hb, resulting in a change in his affinity for O2. This stimulates oxidative modification of Hb, which is reflected in the increasing accumulation of metHb and loss of activities of catalase and GP, and at high concentrations of nitrite to increase the activity of GP.

 эритроциты гемоглобин каталаза глутатионпероксидаза ПОЛ нитрит натрия erythrocytes hemoglobin catalase glutathionperoxidase LPO sodium nitrite

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