Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54278 Общая биофизика General biophysics Общая биофизика Modification of the Incubation Medium Oxidative Activity with Electrochemically Reduced Water Modification of the Incubation Medium Oxidative Activity with Electrochemically Reduced Water Pogorelov A G Pogorelov A G agpogorelov@rambler.ru Pogorelova V N Pogorelova V N Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru 25 06 2018 25 06 2018 3 2 268 273 20 06 2018 20 06 2018 https://rusjbpc.ru/en/nauka/article/54278/view

It has been shown that Dulbecco’s incubation medium prepared with electrochemically reduced water compensates for oxidative stress induced by hydrogen peroxide addition (H2O2, 0.2 mM) to the incubation medium. The incubation medium modified in this way does not affect the H2O2 induced apoptosis in an early mouse embryo. The embryonic cell in the experimental model of apoptosis shows the presence of characteristic morphological changes and decreased cell volume. These apoptosis-related changes were detected using laser-scanning microtomography.

It has been shown that Dulbecco’s incubation medium prepared with electrochemically reduced water compensates for oxidative stress induced by hydrogen peroxide addition (H2O2, 0.2 mM) to the incubation medium. The incubation medium modified in this way does not affect the H2O2 induced apoptosis in an early mouse embryo. The embryonic cell in the experimental model of apoptosis shows the presence of characteristic morphological changes and decreased cell volume. These apoptosis-related changes were detected using laser-scanning microtomography.

 electrochemically reduced water redox potential early mouse embryo apoptosis hydrogen peroxide quantitative laser microtomography electrochemically reduced water redox potential early mouse embryo apoptosis hydrogen peroxide quantitative laser microtomography

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