Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54283 Моделирование в биофизике Modelling in biophycis Моделирование в биофизике MODELING POTEXVIRUSES SPATIAL STRUCTURE МОДЕЛИРОВАНИЕ ПРОСТРАНСТВЕННОЙ СТРУКТУРЫ ПОТЕКСВИРУСОВ Богачева Е Н Bogacheva E N bogen_chph@mail.ru Долгов А А Dolgov A A Ксенофонтов А Л Ksenofontov A L ksenofon@belozersky.msu.ru Институт химической физики им. Н.Н. Семенова РАН ru N.N. Semenov Institute of Chemical Physics RAS ru Институт химической физики им. Н.Н. Семенова РАН ru N.N. Semenov Institute of Chemical Physics RAS ru Московский государственный университет им. М.В. Ломоносова ru Belozersky Institute of Physico-Chemical Biology of Moscow State University ru 25 06 2018 25 06 2018 3 2 296 301 20 06 2018 20 06 2018 https://rusjbpc.ru/en/nauka/article/54283/view

Метод тритиевой планиграфии нашел применение к решению широкого круга задач современной биофизики, структурной химии, молекулярной и физико-химической биологии. Он основан на неселективном замещении водорода в углеводородных фрагментах молекул на его радиоактивный изотоп - тритий. Данные, полученные этим методом, в сочетании с разработанным нами имитационным алгоритмом тритиевой бомбардировки использованы для получения информации о пространственной организации белков и их комплексов. На примере потексвируса картофеля X показана методика определения ориентации субъединиц в вирусе относительно оси спирали и их укладки при известной структуре субъединицы с учетом пространственных ограничений и экспериментальных данных тритиевой планиграфии. Это позволяет детализировать четвертичную структуру вируса и существенно сократить выбор возможных ориентаций субъединиц в вирионе.

The method of tritium planigraphy has applied to solving a wide range of problems of modern biophycis, structural chemistry, molecular and physico-chemical biology. It is based on a non-selective substitution of hydrogen in hydrocarbon fragments of molecules for its radioactive isotope-tritium. The data obtained by this method, in combination with our imitation algorithm of tritium bombardment, was successfully used to obtain information on the spatial organization of proteins and their complexes. Details of the structure of the potexviruses are currently unknown. The example of potato virus X shows a technique for determining the orientation of subunits in the virus relative to the axis of the helix and their placement under a known subunit structure, taking into account spatial limitations and experimental data of tritium planigraphy. This allows us to detail the quaternary structure of the virion and significantly reduce the choice of subunits possible orientations in the virion.

 структура потексвирусов X-вирус картофеля тритиевая планиграфия spatial structure of potato virus X tritium planigraphy

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