Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54289 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ BIFUNCTIONAL INDICATOR SYSTEM FOR PHENOL AND GLUCOSE DETECTION BASED ON NANODIAMONDS AND EXTRACELLULAR FUNGAL OXIDASES БИФУНКЦИОНАЛЬНАЯ ИНДИКАТОРНАЯ СИСТЕМА ДЛЯ ОПРЕДЕЛЕНИЯ ФЕНОЛА И ГЛЮКОЗЫ НА ОСНОВЕ НАНОАЛМАЗОВ И ЭКСТРАКЛЕТОЧНЫХ ГРИБНЫХ ОКСИДАЗ Ронжин Н О Ronzhin N O roniol@mail.ru Могильная О. А. Mogilnaya O. A. ol_mog@mail.ru Артеменко К С Artemenko K S Бондарь В С Bondar V S Институт биофизики Федерального исследовательского центра “Красноярский научный центр Сибирского отделения РАН” ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS” ru ФИЦ КНЦ СО РАН Красноярск Россия FRC KSC SB RAS Krasnoyarsk Russian Federation Институт биофизики Федерального исследовательского центра “Красноярский научный центр Сибирского отделения РАН” ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS” ru Институт биофизики Федерального исследовательского центра “Красноярский научный центр Сибирского отделения РАН” ru Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Federal Research Center “Krasnoyarsk Science Center SB RAS” ru 25 06 2018 25 06 2018 3 2 331 337 20 06 2018 20 06 2018 https://rusjbpc.ru/en/nauka/article/54289/view

С помощью адсорбции экстраклеточных оксидаз (глюкозооксидаза и пероксидазы) светящегося гриба Neonothopanus nambi на модифицированные наноалмазы (МНА) детонационного синтеза сконструирована бифункциональная индикаторная система для обнаружения глюкозы и фенола в водной среде. Установлено, что изучаемые ферменты прочно связываются частицами МНА и проявляют после этого каталитическую активность. В модельных экспериментах in vitro показано, что полученная индикаторная система (комплекс МНА-ферменты) может многократно использоваться для тестирования глюкозы и фенола и проявляет функциональную активность в течение длительного (до 1 месяца) хранения при 4 °С. Полученные данные открывают перспективы конструирования на основе частиц МНА и ферментов высших грибов нового класса многоразовых индикаторных тест-систем для медицинской и экологической аналитики.

A bifunctional indicator system for glucose and phenol detection has been constructed by adsorption of extracellular oxidases (glucose oxidase and peroxidases) of luminous fungus Neonothopanus nambi onto modified nanodiamonds (MND) synthesized by detonation. It has been found that the studied enzymes are strongly bound by MND particles and exhibit catalytic activity. Model experiments in vitro have shown that created indicator system (MND-enzymes complex) can be repeatedly used for glucose and phenol testing and exhibit their activity after storage at 4 °C for a long time (up to 1 month). The data obtained open the prospects for developing a new class of reusable multifunctional indicating and diagnostic test-systems on the basis of MND particles and higher fungi enzymes for medical and ecological analytics.

 наноалмазы светящийся гриб мицелий β-глюкозидаза глюкозооксидаза пероксидаза индикаторная система nanodiamonds luminous fungus mycelium β-glucosidase glucose oxidase peroxidase indicator system

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