Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54306 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MODELING PROTEASE ACTIVITY USING THE ELECTROCHEMICAL INTERFACE МОДЕЛИРОВАНИЕ ПРОТЕАЗНОЙ АКТИВНОСТИ С ИСПОЛЬЗОВАНИЕМ ЭЛЕКТРОХИМИЧЕСКОГО ИНТЕРФЕЙСА Черенков И А Cherenkov I A ivch75@yandex.ru Раевских К С Raevskih K S Сергеев В Г Sergeyev V G Кривилев М Д Krivilev M D Удмуртский государственный университет ru Udmurtia State University ru Удмуртский государственный университет ru Udmurtia State University ru Удмуртский государственный университет ru Udmurtia State University ru Удмуртский государственный университет ru Udmurtia State University ru 25 06 2018 25 06 2018 3 2 422 426 20 06 2018 20 06 2018 https://rusjbpc.ru/en/nauka/article/54306/view

Предложена биоэлектрохимическая модель для оценки протеазной активности, основанная на детекции токов восстановления электроактивного соединения (толуидинового синего), диффундирующего в среде желатинового геля. Электрохимическое поведение толуидинового синего в желатиновом геле характеризуется ростом пиковых значений силы тока во времени и сдвигом потенциалов пиковых значений силы тока относительно показателей, полученных в фосфатно-солевом буферном растворе. Внесение в ячейку трипсина в концентрации 0,08 % (масс.) привело к изменению характера зависимости «ток-время», отражающему снижение диффузионных ограничений. Показателями протеазного катализа в данной модели стали значения силы тока восстановления и скорость изменения силы тока. Наиболее существенные изменения вольтамперных кривых отмечены к 10-й минуте эксперимента. Развитием описанной модели может стать электрохимический сенсор протеазной активности для оценки ферментных препаратов и клеточных культур.

A bioelectrochemical model for assessment of protease activity which based on detection of reduction currents of electroactivity compounds (toluidine blue), diffusing through gelatin gel, was come up. The electrochemical behavior of toluidine blue in gelatin gel is characterized by increase in the peak values of current in time and shift in potential of peak values of the current relative to the parameters obtained in the phosphate buffered saline. Introduction of trypsin in the electrochemical cell in concentration of 0,08 % (wt.) led to a change in nature of current-time relationship, which reflects a decrease in diffusion limitation. An indicator of protease catalysis in this model were the values of the reduction current and the rate of change in the current. The most significant changes in the current-potential curve were observed by the 10th minute of experiment. The development of the described model can be an electrochemical sensor of protease activity for the evaluation of enzyme preparations and cell cultures.

 циклическая вольтамперометрия трипсин фенотиазиновые красители гидрогели cyclic voltammetry trypsin phenothiazine dyes hydrogels

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