Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54332 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ THE FEATURES OF TUMORAL 2-DEOXY-D-RIBOSE-1-PHOSPHATE GENERATION, ASSOCIATION WITH PRODUCTION OF REACTIVE OXYGEN SPECIES ОСОБЕННОСТИ ГЕНЕРАЦИИ 2-ДЕЗОКСИ-D-РИБОЗО-1-ФОСФАТА ОПУХОЛЬЮ, СВЯЗЬ С ПРОДУКЦИЕЙ АКТИВНЫХ ФОРМ КИСЛОРОДА Бакурова Е. М. Bakurova E. M. 32023@mail.ru Донецкий национальный медицинский университет им. М. Горького Донецк Россия Maksim Gorky National Medical University Donetsk Russian Federation 25 09 2018 25 09 2018 3 3 584 587 20 09 2018 20 09 2018 https://rusjbpc.ru/en/nauka/article/54332/view

Выполнено сравнительное исследование активности ферментов обмена тимидина, аденозина и антиоксидантной системы в 44 образцах тканей эпителиальных опухолей распространенного рака желудка и кишечника (ЖКТ), немелкоклеточного рака легких (НМКРЛ). В качестве индивидуального контроля использовали нетрансформированные ткани края резекции. Выявлено повышение опухолевой активности тимидинфосфорилазы (ТФ) в 1,8 раза (p = 0,002) и аденозиндезаминазы (АДА) в 1,7 - 1,9 раз (p = 0,001); установлена корреляция трансферазной активности ТФ и АДА (ρ = 0,704; р < 0,001). Это обеспечивает как продукцию 2-дезокси-D-рибозо-1-фосфата (2-д-D-Риб-1-Ф), так и ресинтез дезокситимидина, необходимого для синтеза дезокситимидилата по «запасному пути». При этом снижение активности глутатионпероксидазы способствует как усилению процессов гликирования в опухолях избыточной 2-д-D-Риб, так и повышению уровней Н2О2. Сделан вывод, что данные особенности обмена способствуют резонированию эффектов гликативного и оксидативного стрессов, сопровождающих опухолевую прогрессию.

A comparative study of the enzymes activity of thymidine, adenosine metabolism and the activity of antioxidant defense enzymes in tumors of different localizations was carried out. Aim: to evaluate the relevance of thymidine phosphorylase (TP), adenosine deaminase (ADA) in 2-deoxy-D-ribose (2-d-D-R-1-P) formation and their association with superoxide dismutase, glutathione peroxidase activity in tumors. We demonstrated that tumor TP activity is higher by 1,8 times (p = 0,002), ADA activity is higher by 1,7-1,9 times (p = 0,001) as compared to non-neoplastic tissues of resection margin. The positive correlation between the intensity of TP and ADA activity was shown (the index of Spearman’s rank correlation, i.e. ρ = 0,704; р < 0,001). So, tumors can increase the levels of 2-deoxy-D-ribose (2-d-D-R) and can stimulate of deoxythymidilate synthesis by the «salvage pathway» in one time. The nucleoside enzymes activity changes being accompanied by decrease of glutathione peroxidase activity in the tumors. Conclusion: It could be suggested that in cancers of different localizations the glycation by 2-d-D-R being associated with hydrogen peroxide formation. Here, we provide evidence on the impact and cooperation of glycative stress and oxidative stress in promoting human cancer progression.

 ферменты 2-дезокси-D-рибоза перекись водорода опухоль enzymes 2-deoxy-D-ribose hydrogen peroxide cancer

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