Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54343 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ INVESTIGATION OF RNA-PROTEIN INTERACTION BETWEEN sRNA rdlD AND THE ESCHERICHIA COLI RNA CHAPERONE PROQ AS WELL AS ITS ISOLATED DOMAINS ИССЛЕДОВАНИЕ ВЗАИМОДЕЙСТВИЯ РНК-ШАПЕРОНА PROQ ИЗ ESCHERICHIA COLI И ЕГО ДОМЕНОВ C МАЛОЙ РЕГУЛЯТОРНОЙ РНК rdlD Леконцева Н В Lekontseva N V natalja-lekontseva@rambler.ru Фандо М С Fando M S Михайлина А О Mikhailina A O Коробейникова А В Korobeinikova A V Никулин А Д Nikulin A D Институт белка РАН ru Institute of protein research RAS ru Институт белка РАН ru Institute of protein research RAS ru Институт белка РАН ru Institute of protein research RAS ru Институт белка РАН ru Institute of protein research RAS ru Институт белка РАН ru Institute of protein research RAS ru 25 09 2018 25 09 2018 3 3 651 653 20 09 2018 20 09 2018 https://rusjbpc.ru/en/nauka/article/54343/view

Для поддержания стабильности и правильного функционирования мрРНК часто необходимы РНК-шапероны - РНК-связывающие белки, способные плавить вторичную структуру РНК. Недавние исследования показали, что белки, содержащие домен ProQ/FinO, можно выделить в новый класс РНК-шаперонов, которые могут играть ключевую роль в регуляции трансляции ряда генов. Для исследования механизмов РНК-белкового узнавания данным классом белков нами выбран белок ProQ из Escherichia coli . Мы показали, что он способен специфически взаимодействовать с мрРНК rdlD, причем N- и С-концевые домены белка имеют разное сродство к исследуемой РНК.

The stability and function of sRNAs are often determined by specialized RNA-binding proteins called «RNA chaperones» which can melt RNA secondary structure. Recently has been found ProQ/FinO domain constitute a new class of RNA chaperones that can play key roles in post-transcriptional gene regulation. To study molecular mechanisms of the RNA-protein interactions carried out by this class of proteins, ProQ from Escherichia coli has been chosen. We have shown that the protein can specifically interact with rdlD sRNA and its N- and C-terminal domains possess different affinity to the sRNA.

 ProQ малые регуляторные РНК РНК-шапероны регуляция трансляции sRNAs RNA chaperones translation regulation

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