Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54355 Общая биофизика General biophysics Общая биофизика OXYGEN TENSION IN BLOOD, SKELETAL MUSCLE AND PECULIARITIES OF TISSUE METABOLISM IN LIZA AURATA (RISSO, 1810) UNDER CONDITIONS OF ACUTE EXPERIMENTAL HYPOXIA НАПРЯЖЕНИЕ КИСЛОРОДА В КРОВИ, СКЕЛЕТНЫХ МЫШЦАХ И ОСОБЕННОСТИ ТКАНЕВОГО МЕТАБОЛИЗМА У КЕФАЛИ-СИНГИЛЯ (LIZA AURATA RISSO, 1810) В УСЛОВИЯХ ОСТРОЙ ЭКСПЕРИМЕНТАЛЬНОЙ ГИПОКСИИ Солдатов А А Soldatov A A alekssoldatov@yandex.ru Институт морских биологических исследований им. А.О. Ковалевского РАН ru Institute of Marine Biological Research A.O. Kovalevsky ru 25 12 2018 25 12 2018 3 4 724 729 20 12 2018 20 12 2018 https://rusjbpc.ru/en/nauka/article/54355/view

В условиях острого эксперимента исследовано влияние ранжированной гипоксии на напряжение кислорода в артериальной, венозной крови ( PaO2 , PvO2 ) и скелетных мышцах ( PmO2 ) кефали-сингиля ( Liza aurata , Risso, 1810). Одновременно по содержанию лактата и величинам рН в крови и мышечной ткани оценен уровень анаэробных процессов в скелетных мышцах рыб. Контрольная группа кефалей содержалась при напряжении кислорода 160-162 гПа, температуре - 14-16 оС и фотопериоде: 12 часов день - 12 часов ночь. Экспериментальные группы рыб выдерживались в течение 15-ти суток при 85, 55 и 35 гПа при сходной температуре воды и фотопериоде. Напряжение кислорода снижали посредством барботажа воды азотом. Наиболее выраженные изменения отмечали при адаптации рыб к 55 и 35 гПа. Величины PvO2 и PmO2 (1-е сутки) понижались соответственно на 28-33 и 19-31 % (p<0,01). Параллельно происходил рост содержания лактата, в большей степени в красной мышечной ткани, и снижение значений рН венозной крови, оттекающей от скелетных мышц, что отражало усиление анаэробных процессов. Содержание особей кефали при 55 гПа в течение 10-15-ти суток приводило к почти полной компенсации отмеченных выше изменений. Концентрация лактата в мышцах, PvO2 и рН крови восстанавливались до уровня контрольных значений. При 35 гПа нормализации кислородного режима в мышечной ткани не наблюдали, что сопровождалось гибелью посадочного материала на 3-и сутки эксперимента.

The effect of ranked hypoxia on the oxygen tension in arterial blood, venous blood ( PaO2, PvO2 ) and skeletal muscles ( PmO2 ) of mullet ( Liza aurata , Risso, 1810) was studied under the conditions of acute experiment. At the same time, the level of anaerobic processes in the skeletal muscles of fish was estimated basing on the lactate content and pH values in blood and muscle tissue. The control group of mullets was maintained under the oxygen tension of 160-162 kPa, temperature of 14-16оС and photoperiod of 12 hours of light: 12 hours of darkness. Experimental groups of fish were kept for 15 days at 85, 55 and 35 kPa at a similar water temperature and photoperiod. Oxygen tension was reduced by bubbling water with nitrogen. The most pronounced changes were observed during the adaptation of fish to 55 and 35 kPa. The values of PvO2 and PmO2 (first day) decreased by 28-33 and 19-31 %, respectively (p<0.01). In parallel, there was an increase in the lactate content, to a greater extent in the red muscle tissue, and a decrease in the pH of venous blood flowing from the skeletal muscles, which reflected the intensification of the anaerobic processes. Maintaining of a mullet at 55 kPa for 10-15 days resulted in nearly complete compensation of the changes mentioned above. The concentration of lactate in the muscles, PvO2 and blood pH was restored to the level of control values. At 35 kPa the normalization of the oxygen regime in the muscle tissue was not registered and the mullets died on the 3rd day of the experiment.

 гипоксия напряжение кислорода скелетные мышцы кровь лактат рН Liza aurata hypoxia oxygen tension skeletal muscles blood lactate pH Liza aurata

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