Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54373 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ POLYMERIC MICELLES FOR DEXAMETHASONE DELIVERY ПОЛИМЕРНЫЕ МИЦЕЛЛЫ ДЛЯ ДОСТАВКИ ДЕКСАМЕТАЗОНА Коржикова-Влах Е Г Korzhikova-Vlakh E G vlakh@mail.ru Вдовченко А А Vdovchenko A A Стулова Е Г Stulova E G Зашихина Н Н Zashikhina N N Левит М Л Levit M L Тенникова Т Б Tennikova T B Санкт-Петербургский государственный университет; Институт высокомолекулярных соединений Российской академии наук ru Saint-Petersburg State University; Institute of Macromolecular Compounds of Russian Academy of Sciences ru Санкт-Петербургский государственный университет ru Saint-Petersburg State University ru Санкт-Петербургский государственный университет ru Saint-Petersburg State University ru Институт высокомолекулярных соединений Российской академии наук ru Institute of Macromolecular Compounds of Russian Academy of Sciences ru Институт высокомолекулярных соединений Российской академии наук ru Institute of Macromolecular Compounds of Russian Academy of Sciences ru Санкт-Петербургский государственный университет ru Saint-Petersburg State University ru 25 12 2018 25 12 2018 3 4 824 830 20 12 2018 20 12 2018 https://rusjbpc.ru/en/nauka/article/54373/view

Полимерные наночастицы различного состава и морфологии находят все более широкое применение для создания систем доставки лекарств. В данной работе обсуждается получение полимерных мицелл на основе амфифильных сополимеров, таких как поли( N -метакриламидо- D -глюкоза)- б -поли( О -холестерилметакрилат) и поли( L -лизин- со - L -фенилаланин) с различным соотношением аминокислот. Указанные сополимеры были синтезированы с использованием метода контролируемой полимеризации с обратимой передачей цепи и метода полимеризации с раскрытием цикла N -карбоксиангидридов a-аминокислот, соответственно, и охарактеризованы методами ГПХ, ЯМР и аминокислотного ВЭЖХ анализа. На основе синтезированных амфифильных сополимеров были получены сферические наночастицы, гидродинамический диаметр которых лежал в пределах от 110 до 200 нм в зависимости от состава сополимера. Морфология полученных наночастиц была исследована методом просвечивающей электронной микроскопии. Для всех синтезированных полимеров наблюдалось формирование сферических мицелл. Эффективность инкапсулирования дексаметазона в полимерные мицеллы составила 70-90 %. Полученные системы характеризовались стабильностью в отношении самопроизвольного высвобождения дексаметазона в модельных физиологических условиях.

Polymer nanoparticles of various composition and morphology are increasingly used to create drug delivery systems. This paper discusses the preparation of polymeric micelles based on amphiphilic copolymers such as poly ( N -methacrylamido- D -glucose)- b -poly( O -cholesteryl methacrylate) and poly ( L -lysine- co - L -phenylalanine) with different amino acid ratios. These copolymers were synthesized using a controlled radical polymerization and a ring-opening polymerization of N-carboxyanhydrides of a-amino acids, respectively, and characterized by GPC, NMR and amino acid HPLC analysis. Based on the synthesized amphiphilic copolymers, spherical nanoparticles were obtained whose hydrodynamic diameter ranged from 110 to 200 nm, depending on the composition of the copolymer. The morphology of the obtained nanoparticles was studied by transmission electron microscopy. For all synthesized polymers, the formation of spherical micelles was observed. The efficiency of dexamethasone encapsulation in polymeric micelles was 70-90 %. The obtained systems were characterized by stability with respect to the spontaneous release of dexamethasone under model physiological conditions.

 амфифильные сополимеры наночастицы полимерные мицеллы инкапсулирование лекарств дексаметазон amphiphilic copolymers nanoparticles polymer micelles drug encapsulation dexamethasone

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