Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54413 Общая биофизика General biophysics Общая биофизика THE INITIATED OXIDATION OF PHOSPHATIDYLCHOLINE LIPOSOMS AND CHANGE OF THEIR PHYSICAL AND CHEMICAL CHARACTERISTICS IN THE COURSE OF OXIDATION ИНИЦИИРОВАННОЕ ОКИСЛЕНИЕ ФОСФАТИДИЛХОЛИНОВЫХ ЛИПОСОМ И ИЗМЕНЕНИЕ ИХ ФИЗИКО-ХИМИЧЕСКИХ ХАРАКТЕРИСТИК В ПРОЦЕССЕ ОКИСЛЕНИЯ Сажина Н Н Sazhina N N atnik48s@yandex.ru Пальмина Н П Palmina N P Плащина И Г Plashchina I G Антипова А С Antipova A S Семенова М Г Semenova M G Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics RAS ru 25 06 2019 25 06 2019 4 2 151 159 20 06 2019 20 06 2019 https://rusjbpc.ru/en/nauka/article/54413/view

Изучена кинетика инициированного 2,2’- азобис (амидинопропан) дигидрохлоридом (ААРН) окисления липосом из соевого фщсфатидилхолина (ФХ) при разных температурах и с добавлением в них альфа-линоленовой кислоты (АЛК), а также ингибирования окисления этих липосом введением в них эфирного масла гвоздики (ЭМГ) и инкапсулированием их казеинатом натрия (КН). Методом динамического рассеяния света (ДСР) зарегистрирована динамика изменения размеров и z-потенциала липосом в процессе их окисления. Результаты работы показали, что инициированное ААРН окисление липосом из соевого ФХ при Т = 370С можно достаточно эффективно (на 80-90%) ингибировать фенольными соединениями ЭМГ. При повышении температуры до 600С в 4-5 раз увеличивается скорость окисления липосом, и ингибирование образования продуктов ПОЛ существенно уменьшается. Добавление в ФХ-липосомы АЛК приводит к ускорению окисления по сравнению с ФХ-липосомами. Инкапсулирование липосом казеинатом натрия снижает окисление липосом на 70%, а совместное действие его с ЭМГ - на 90%. С повышением температуры эффективность защитной функции казеиновой оболочки уменьшается. В процессе окисления при Т = 370С размер ФХ-липосом увеличивается на 6-7%, а абсолютное значение отрицательного z-потенциала увеличивается к концу окисления примерно в 3 раза. Введение в липосомы АЛК значительно влияет на динамику изменения их размеров и z-потенциала. Присутствие в липосомах ЭМГ стабилизирует размеры и z-потенциал в процессе окисления и для ФХ-липосом, и для липосом с АЛК. Комплекс ФХ-липосом с казеинатом натрия также приводит к стабилизации его размеров и z-потенциала.

In the present work the kinetics of initiated by awater-soluble azo-initiator AAPH oxidation of liposomes from soy phosphatidylcholine (PC) at different temperatures and with addition in them of the alpha-linolenic acid (ALA) and also inhibition of liposome oxidation by essential oil of clove buds (EOC) and liposome encapsulation in sodium caseinate (SC) is studied. Dynamics of change of liposome sizes and z-potential in the course of their oxidation is registered by the method of dynamic light scattering. Results of work showed that the liposome oxidation initiated by AAPH from soy PC at T = 370C is inhibited effectively (80-90%) by EOC phenolic connections. At temperature increase to 600C the speed of liposome oxidation increases by 4-5 times, and the inhibition of formation of oxidation products significantly decreases. Addition in PC liposomes of ALA leads to acceleration of oxidation in comparison with PC liposomes. Encapsulation of liposomes by SC reduces liposome oxidation by 70%, and its joint action with EOC - by 90%. With temperature increase decreases the efficiency of protective function of SC cover. In the course of oxidation at T = 370C the size of PC-liposomes increases by 6-7%, and the absolute value of negative z-potential increases in oxidation end approximately by 3 times. Introduction to liposomes of ALA considerably affects dynamics of change of their sizes and z-potential. Presence of EOC in liposomes stabilizes their sizes and z-potential in the course of oxidation both for PC liposomes, and for liposomes with ALA. The complex of PC-liposomes with SC also leads to stabilization of its sizes and z-potential.

 липосомы ПОЛ фосфатидилхолин эфирное масло гвоздики казеин натрия альфа-линоленовая кислота liposomes lipid peroxidation phosphatidylcholine essential oil of clove sodium casein alpha-linolenic acid

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