Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54463 Общая биофизика General biophysics Общая биофизика MEASUREMENT OF ABSORPTION SPECTRUM OF DENSE CULTURES OF BENTHIC MICROALGAE ИЗМЕРЕНИЕ СПЕКТРА ПОГЛОЩЕНИЯ ПЛОТНЫХ КУЛЬТУР БЕНТОСНЫХ МИКРОВОДОРОСЛЕЙ Железнова С Н Zheleznova S N Малахов А С Malakhov A S mac89@mail.ru Геворгиз Р Г Gevorgiz R G r-gevorgiz@yandex.ru Институт биологии южных морей им. А.О. Ковалевского РАН ru A.O. Kovalevsky Institute of Marine Biological Research of RAS ru Национальный исследовательский Томский политехнический университет ru National research Tomsk Polytechnic University ru Институт биологии южных морей им. А.О. Ковалевского РАН ru A.O. Kovalevsky Institute of Marine Biological Research of RAS ru 25 12 2019 25 12 2019 4 4 463 466 20 12 2019 20 12 2019 https://rusjbpc.ru/en/nauka/article/54463/view

В работе рассматриваются проблемы измерения спектров поглощения бентосных видов диатомовых водорослей. При измерениях спектров культур бентосных микроводорослей ошибкам, связанным с рассеиванием света, сопутствуют ошибки, связанные с оседанием клеток. Показано, что использование портативных спектрофотометров при ориентации оптического пути в вертикальном положении скорость оседания бентосных водорослей не влияет на измерение спектров поглощения в области ФАР. Сообщается о необходимости учитывать спектры флюоресценции культуры из-за наличия большой доли флюоресценции в красной области.

In this paper, the problems in measuring the absorption spectra of benthic diatom were considered.When measuring the spectra of cultures of benthic microalgae, there are errors associated with light scattering and associated with the subsidence of cells. It is shown that the use of portable spectrophotometers when orienting the optical path in a vertical position does not affect the measurement of absorption spectra in the PAR region. It is reported that it is necessary to take into account the fluorescence spectra of the culture due to the presence of a large proportion of fluorescence in the red region.

 микроводоросли бентосные диатомовые водоросли диатомовая водоросль Cylindrotheca closterium microalgae benthic diatoms diatom Cylindrotheca closterium

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