Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54486 Общая биофизика General biophysics Общая биофизика The influence of phytocompositions dandelion-burdock on electrophysiological characteristics of Chara fragilis cells Влияние фитокомпозиций одуванчик-лопух на электрофизиологические характеристики клеток Chara fragilis Гасанова А. Э. Hasanova A. E. ahasanova89@gmail.com Бакинский государственный университет Баку Азербайджан Baku State University Baku Azerbaijan Институт ботаники НАН Азербайджана Баку Россия Institute of Botany ANAS Baku Russian Federation 25 03 2020 25 03 2020 5 1 29 34 20 03 2020 20 03 2020 https://rusjbpc.ru/en/nauka/article/54486/view

С использованием микроэлектродных методов были изучены закономерности изменения потенциала (φm) и сопротивления (Rm) плазматической мембраны (ПM) клеток Chara fragilis под влиянием растворов фитокомпозиции Одуванчик - Лопух (КОЛ). Представлены результаты анализа распределения потенциала φm, сопротивления Rm плазматической мембраны по количеству клеток Chara fragilis в стандартных условиях. Среднее значение этих параметров в стандартных условиях составило -183 ± 4,9 мВ и 9 ± 1,2 Ом·м2.Для действия низких концентраций КОЛ (0,01 мг / мл) в диапазоне активации К+- каналов наружного выпрямления φm была характерна значительная деполяризация ПM.В работе обсуждается ингибирование транспорта ионов через ПМ с точки зрения окисления и изменений в физическом состоянии его липидной фазы.

By using of microelectrode techniques, patterns of change in the potential (φm) and resistance (Rm) of the plasma membrane (PM) of Chara fragilis cells under the influence of Dandelion-Burdock (DBC) phytocomposition solutions were studied. It is important to emphasize that the results of the analysis of the potential distribution, resistance of the plasma membrane by the number of cells of Chara fragilis under the standard conditions is being presented for the first time. The Hogg method allows simultaneous measuring of electrophysiological parameters such as φm and Rm. The average value of these parameters were -183±4.9 mV and 9±1.2 Оhm·m2 under the standard conditions. For the action of low DBC concentrations (0.01 mg/ml) in the activation range of the KCOR was characterized by significant depolarization of PM. In this paper, the inhibition of ion transport through the PM is discussed from the point of view of oxidation, and hyperpolarization-changes in the physical state of its lipid phase.

 Chara fragilis мембранный потенциал мембранное сопротивление фитопротекторы Н+- насосы К+- каналы Chara fragilis membrane potential membrane resistance phytoprotectors H+ - pumps K+ - channels

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