Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54500 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Resveratrol increases the body's resistance to stressful exposures Ресвератрол повышает устойчивость организма к стрессовым воздействиям Жигачева И. В. Zhigacheva I. V. zhigacheva@mail.ru Русина И Ф Rusina I F rusina939@mail.ru Васильева С В Vasilyeva S V Петрищева М С Petrishcheva M S Институт биохимической физики им. Н.М. Эмануэля РАН Москва Россия N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences Moscow Russian Federation Институт химической физики им Н.Н. Семенова РАН ru N.N. Semenov Institute of Chemical Physics Russian Academy of Sciences ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute of Biochemical Physics. Russian Academy of Sciences ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute of Biochemical Physics. Russian Academy of Sciences ru 25 03 2020 25 03 2020 5 1 130 139 20 03 2020 20 03 2020 https://rusjbpc.ru/en/nauka/article/54500/view

Изучена антиоксидантная и антирадикальная активность природного фитоалексина - ресвератрола (РВ) в сравнении с антирадикальной и антиоксидантной активностью известных антиоксидантов. Препарат обладает довольно высокими скоростями взаимодействия с пероксильными радикалами. Показано, что при низких концентрациях [РВ] £10-5M ресвератрол проявлял свойства сильного ингибитора свободно-радикального окисления. В концентрациях [РВ]³10-5M активировал свободно-радикальное окисление. Исследование антиоксидантной активности РВ на модели «старения» митохондрий показало наличие у препарата высокой антиоксидантной активности. РВ предотвращал активацию ПОЛ в мембранах митохондрий печени крыс при более низких концентрациях, чем известные антиоксиданты ионол (дибунол) и мексидол. Высокая антирадикальная и антиоксидантная активность ресвератрола, вероятно, могла обеспечивать наличие у препарата антистрессовых свойств. Исследование проявления антистрессовых свойств проводили на молекулярно - генетической модели E.coli. Испытание различных доз и композиций РВ с такими биологически активными соединениями как донор NO - ТНКЖ-тио, тиомочевина (TМ) и мексидол показало, что совместная комплексная обработка клеток E.coli РВ /TМ и донором NO - ТНКЖ-тио почти в 4-6 раз усиливала экспрессию генов SOS - и SoxRS - ДНК- репарационных систем защиты. Исследование адаптогенных свойств препарата на моделях острого алкогольного отравления (ОАО) и острой гипобарической гипоксии (ОГГ) для крыс, а также дефицита воды (ДВ) для проростков гороха подтвердило наличие у препарата РВ этих свойств. Введение животным 10-9М РВ за 45 минут до ОАО или ОГГ предотвращало активацию ПОЛ. Кроме того, обработка семян гороха 3×10-5М РВ предупреждала активацию ПОЛ в мембранах митохондрий проростков гороха в условиях дефицита воды. На основании полученных данных можно предположить, что адаптогенные свойства ресвератрола, вероятно, обусловлены его антиоксидантной и антирадикальной активностью.

The antioxidant and antiradical activity of natural phytoalexin - resveratrol (RV) was studied in comparison with the antiradical and antioxidant activity of known antioxidants. The preparation possessed rather high values of the rate constant of interaction with peroxyl radicals. Resveratrol was shown to exhibit the properties of a strong inhibitor of free radical oxidation at low concentrations of [RV] £ 10-5M. In concentrations exceeding [RV]³10-5M , the RV exhibited prooxidant properties. A study of the antioxidant activity of RV on the model of mitochondrial “aging” demonstrated the presence of a high antioxidant activity at the drug. RV prevented the activation of LPO in the membranes of rat liver mitochondria at lower concentrations, than the known antioxidants ionol (dibunol) and mexidol. The presence of high antiradical and antioxidant activity in resveratrol probably could indicate the presence of anti-stress properties in the drug. Verification for these properties was carried out using the molecular genetic model of E. coli . Testing of various doses and combinations of RV with biologically active compounds such as a NO donor - TNIC-thio, thiourea (TU) and mexidol showed that the combined treatment of E. coli cells with RV / TU and a NO donor - TNIC-thio is almost 4-6 times enhanced the expression of the SOS and SoxRS genes of DNA of repair systems of defense. The study of the adaptogenic properties of the drug on models of acute alcohol poisoning (AAP) and acute hypobaric hypoxia (AHH) for rats, as well as water deficit (WD) for pea seedlings confirmed the presence of these properties. The introduction of 10-9 M RV to animals 45 minutes before AAP or AHH prevented the activation of LPO. In addition, the treatment of pea seeds 3×10-5M RV prevented the activation of LPO in the membranes of pea seedlings mitochondria in conditions of water deficiency. Based on the data obtained, it can be assumed that the adaptogenic properties of resveratrol are probably due to its antioxidant and anti-radical activity.

 ресвератрол антиоксиданты стресс ПОЛ экспрессия генв митохондрии E. coli resveratrol antioxidants chemiluminescence stress lipid peroxidation gene expression mitochondria E. coli

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