Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54570 Общая биофизика General biophysics Общая биофизика Comparative analysis of different protocols for DNA extraction from Arthrospira platensis Сравнительный анализ различных протоколов выделения ДНК Arthrospira platensis Дегтяр И В Degtyar I V skuratovskaya95@mail.ru Лантушенко А О Lantushenko A O Лелеков А С Lelekov A S Севастопольский государственный университет ru Sevastopol State University ru Севастопольский государственный университет ru Sevastopol State University ru ФИЦ «Институт биологии южных морей им. А.О. Ковалевского РАН» ru A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS ru 25 12 2020 25 12 2020 5 4 587 591 20 12 2020 20 12 2020 https://rusjbpc.ru/en/nauka/article/54570/view

В статье рассмотрены методики выделения ДНК из цианобактерии Arthrospira platensis. Для контроля чистоты и целостности исследуемой культуры использовался микроскопический и спектрофотометрический анализ. Эксперимент по выделению ДНК проводился по четырём различным протоколам: два с использованием колонок, кислотно-щелочной и с помощью набора «ДНК-экстран». Контроль чистоты выделенной ДНК и определение ее концентрации определялось с помощью спектрофотометра Implen NanoPhotometer N60. Наибольшая концентрация выделенной ДНК была получена с использованием кислотно-щелочного метода, при этом наиболее чистые препараты были выделены набором «ДНК-экстран» и на колонках «К-СОРБ».

The article discusses the methods for DNA extraction from cyanobacteria Arthrospira platensis. Microscopic and spectrophotometric analysis was used to control the purity and integrity of the culture under study. The DNA extraction experiment was performed using four different protocols: two using columns, an acid-base one, and a set of"DNA-extran". Control of the purity of the isolated DNA and determination of its concentration was determined using the Implen NanoPhotometer N60 spectrophotometer. The highest concentration of isolated DNA was obtained using the acid-base method, while the purest preparations were isolated using a set of "DNA-extran" and "K-Sorb" columns.

 Arthrospira platensis выделение ДНК спектрофотометрия Arthrospira platensis DNA extraction spectrophotometry Работа была выполнена в рамках госзадания № FEFM-2020-0003.

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