Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54612 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Viral infections affect the functional activity of peripheral blood neutrophils Вирусные инфекции влияют на функциональную активность нейтрофилов периферической крови Федорова Н Д Fedorova N Д Сумбатян Д А Sumbatian D А Стукова М А Stukova M А Иванов А В Ivanov A В Семенова Е В Semenova E В Филатов М В Filatov M В Варфоломеева Е Ю Varfolomeeva E Ю НИЦ «Курчатовский институт» - ПИЯФ; НИЦ «Курчатовский институт» ru Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»; National Research Center “Kurchatov Institute” ru НИЦ «Курчатовский институт» - ПИЯФ ru Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute» ru НИИ гриппа им. А.А. Смородинцева ru Smorodintsev Research Institute of Influenza, Russian Ministry of Health ru Клиника высоких медицинских технологий СПбГУ им. Н.И. Пирогова ru Saint-Petersburg State University Hospital ru НИЦ «Курчатовский институт» - ПИЯФ ru Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute» ru НИЦ «Курчатовский институт» - ПИЯФ ru Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute» ru НИЦ «Курчатовский институт» - ПИЯФ; НИЦ «Курчатовский институт» ru Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»; National Research Center “Kurchatov Institute” ru 25 03 2021 25 03 2021 6 1 115 123 20 03 2021 20 03 2021 https://rusjbpc.ru/en/nauka/article/54612/view

Нейтрофилы - основные клетки врожденного иммунитета. Уничтожение патогенных микроорганизмов осуществляется нейтрофилами посредством фагоцитоза и последующей реализации механизмов генерации активных форм кислорода. Цель настоящей работы - изучение влияния вирусных инфекций на функциональную активность (интенсивность реакции респираторного взрыва) нейтрофилов периферической крови. Исследование проводилось на цельной крови человека методом проточной цитометрии. Проанализированы изменения интенсивности реакции респираторного взрыва при развитии ОРВИ и при бактериальных осложнениях. Определен компонент плазмы крови, предположительно праймирующий нейтрофилы при вирусных инфекциях (белок острой фазы фибриноген). Показано, что нейтрофилы здоровых доноров по-разному реагируют на вакцинацию инактивированными и “живыми” вакцинами против гриппа. Выявлена корреляция между способностью нейтрофилов генерировать активные формы кислорода и титром антител IgA и IgG к SARS-CoV-2 у пациентов с бессимптомным или легким Covid-19. Полученные данные подтверждают влияние вирусных инфекций на функциональную активность нейтрофилов. Кроме того, предложен белок, который может быть ответственным за это влияние.

Neutrophils are the primary cells of the innate immune system. They destroy pathogenic microorganisms carrying out the oxidative stress mechanism through phagocytosis and extracellular traps (NETs). The aim of the present work is the study of the influence of the viral infection on functional activity (respiratory burst reaction intensity) of peripheral blood neutrophils. The study was conducted on the whole human blood using the flow cytometry technique. The changes in respiratory burst reaction intensity during ARVI development and in case of bacterial complications appearance were analyzed. The blood plasma component priming neutrophils in viral infections was determined (acute phase protein fibrinogen). It was shown that healthy donors’ neutrophils react differently to vaccination with inactivated and “alive” influenza vaccines. The fact that neutrophils' capacity to generate reactive oxygen species correlates with the titer of IgA and IgG antibodies to SARS-CoV-2 in patients with asymptomatic or mild Covid-19 was revealed. The obtained data confirm the influence of viral infections on the neutrophils’ functional activity. Also, the protein that might be responsible for this influence was suggested.

 нейтрофилы окислительный стресс респираторные вирусные инфекции проточная цитометрия neutrophils oxidative stress respiratory viral infection flow cytometry Работа поддержана Благотворительным Фондом им. В.Н. Фомичева.

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