Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54627 Общая биофизика General biophysics Общая биофизика Decomposition native absorption spectrum of the culture micro alga Arthrospira (Spirulina) Platensis Разделение нативного спектра поглощения культуры Arthrospira (Spirulina) Platensis Чернышев Д Н Chernyshev D N chernishevd@gmail.com Клочкова В С Klochkova V S Севастопольский государственный университет ru Sevastopol State University ru Севастопольский государственный университет ru Sevastopol State University ru 25 06 2021 25 06 2021 6 2 217 221 20 06 2021 20 06 2021 https://rusjbpc.ru/en/nauka/article/54627/view

В работе проведено математическое разделение спектра поглощения культуры микроводоросли Arthrospira ( Spirulina ) platensis. Спектры культуры регистрировались на спектрофотометре с интегрирующей сферой. При анализе спектра вычислена производная второго порядка и определены основные максимумы поглощения. Полученные данные послужили основой при моделировании. Спектр поглощения культуры микроводоросли A. platensis в области от 400 до 800 нм был описан шестью кривыми Гаусса. Составлена общая модель спектра поглощения культуры из отдельных пигментов. Применяя полученную модель, возможно определить концентрации хлорофилла а , суммарных каротиноидов и фикобилинов непосредственно по спектру поглощения нативной культуры.

The work carried out a mathematical separation of the absorption spectrum of the culture of the microalga Spirulina platensis. Culture spectra were recorded on an integrating sphere spectrophotometer. When analyzing the spectrum, the second-order derivative was calculated and the main absorption maxima were determined. The obtained data served as the basis for modeling. The absorption spectrum of the culture of the microalga Spirulina platensis in the region from 400 to 800 nm was described by six Gaussian curves. A general model of the absorption spectrum of a culture from individual pigments was compiled. Using the resulting model, it is possible to determine the concentration of chlorophyll a, total carotenoids and phycobilins directly from the absorption spectrum of the native culture.

 спирулина каротиноиды хлорофилл а фикобилины спектры поглощения гауссианы Spirulina carotenoids chlorophyll a phycobilins absorption spectra Gaussian curves

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