Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54628 Общая биофизика General biophysics Общая биофизика Effect of NA-ascorbate in the protection of PS II activity under conditions of simultaneous action of CO2+ and photoinhibition Эффект NA-аскорбата в защите активности ФС II в условиях одновременного действия CO2+ и фотоингибирования Ганиева Р А Ganiyeva R A Атакишиева С А Atakishiyeva S A sevincatakisiyeva@gmail.com Гасанов Р А Gasanov R A ra38hasan@gmail.com Институт ботаники Национальной Академии Наук Азербайджана ru Institute of Botany, Azerbaijan National Academy of Sciences ru Институт ботаники Национальной Академии Наук Азербайджана ru Institute of Botany, Azerbaijan National Academy of Sciences ru Бакинский государственный университет ru Baku State University ru 25 06 2021 25 06 2021 6 2 222 225 20 06 2021 20 06 2021 https://rusjbpc.ru/en/nauka/article/54628/view

Функциональное состояние ФС II в листьях проростков пшеницы, подвергнутых комплексному действию Со2+ и фотоингибированию определяли на основании различных характеристик замедленной флуоресценции хлорофилла а (мсек ЗФ Хл а ). Воздействие Со2+ выразилось в резком падении характеристической величины мсек ЗФ Хл а реакционного центра ФС II и в более слабом блокировании донорной стороны характеризующей состояние Mn4O5Ca кластер и Yz. Реактивные формы кислорода, генерируемые в процессе фотоингибирования, блокировали также в большей мере акцепторную сторону ЭТЦ ФС II. Увеличение времени адаптации наблюдалось значительное падение активности на донорной стороне ЭТЦ ФС II. Комплексное воздействие обоих факторов слабо влияло на изменение флуоресцентных характеристик, которые оставались почти на уровне действия Со2+. Показано, что адаптивные возможности фотохимических реакций, протекающих в ЭТЦ ФС II при комплексном стрессе стимулируется низкомолекулярным антиоксидантом Na-аскорбатом. Предполагается, что Na-аск играет решающую роль в защите хлоропластов от окислительного стресса посредством тушения О2¯ и *ОН1.

The functional state of PS II in the leaves of wheat seedlings subjected to the complex action of Co2+ and photoinhibition was determined on the basis of various characteristics of the delayed fluorescence of chlorophyll a (msec DF Chl a ). The effect of Co2+ was expressed in a sharp decrease in the characteristic value of msec DF Chl a of the PS II reaction center and in a weaker blocking of the donor side characterizing the state of the Mn4O5Ca cluster and Yя . Reactive oxygen species (ROS) generated during photoinhibition also blocked to a greater extent the acceptor side of the PS II ETC. An increase in the adaptation time was observed a significant drop in activity on the donor side of the ETC PS II. The combined effect of both factors had little effect on the change in fluorescence characteristics, which remained almost at the level of the effect of Co2+. It has been shown that the adaptive capabilities of photochemical reactions occurring in the ETC of PS II under complex stress are stimulated by the low molecular weight antioxidant Na-ascorbate. It is assumed that Na-asc plays a decisive role in protecting chloroplasts from oxidative stress by quenching O2 ¯ and *OH1.

 ФС II ЭТЦ Со2+ фотоингибирование РФК Na-аскорбат PS II ETC Со2+ photoinhibition ROS Na-ascorbate

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