Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54648 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ The stable radicals for study the enzyme oxidation of phenols Использование стабильных радикалов для изучения кинетики ферментативного окисления фенолов Кравченко Е. М. Kravchenko E. M. elen.kravchenko@donnu.ru Кучина Е С Kuchina E S Лаптева Е. А. Lapteva E. A. Одарюк И. Д. Odaryuk I. D. Донецкий национальный университет Донецк Россия Donetsk National University Donetsk Russian Federation Донецкий национальный университет ru Donetsk National University ru Донецкий национальный университет Донецк Россия Donetsk National University Donetsk Russian Federation Донецкий национальный университет Донецк Россия Donetsk National University Donetsk Russian Federation 25 06 2021 25 06 2021 6 2 325 329 20 06 2021 20 06 2021 https://rusjbpc.ru/en/nauka/article/54648/view

В работе изучена возможность применения стабильных радикалов для определения содержания фенолов в реакционной смеси с помощью окислительно-восстановительного титриметрического анализа. Разработана процедура получения ABTS+•-радикала в реакции лакказного окисления ABTS с последующим выделением катион-радикала гель-хроматографией на колонке с сефадексом G-10. С помощью различных подходов определен коэффициент экстинкции для ABTS+•-радикала, который составил (3,3 ± 0,2)·104 л∙(моль∙см)-1. В качестве реагента для стандартизации раствора ABTS+•-радикала и •DPPH предложена аскорбиновая кислота. Показана применимость окислительно-восстановительного титрования с использованием катион-радикала ABTS и •DPPH для регистрации кинетики расходования многоатомного фенола в процессе его окисления в присутствии лакказы Trametes versicolor .

It was studied the possibility of using stable radicals to determine the content of phenols in the reaction mixture with redox titrimetric analysis. The procedure for obtaining an ABTS+•-radical in the laccase oxidation of ABTS has been developed. The subsequent isolation of the radical cation by gel chromatography on a column with Sephadex G-10 was applied. The extinction coefficient for the ABTS+•-radical was determined using various approaches and it was equaled (3.3±0.2)·104 L∙(mol∙cm)-1. Ascorbic acid has been proposed as a reagent for the solution of ABTS+•-radical and •DPPH standardization. Redox titration with ABTS+• and •DPPH might be used for recording the kinetics of consumption of polyphenol during its oxidation in the presence of the Trametes versicolor laccase.

 стабильные радикалы лакказа фенолы аскорбиновая кислота окисление спектрофотометрия окислительно-восстановительное титрование stable radicals laccase phenols ascorbic acid oxidation spectrophotometry redox titration

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