Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54675 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Effect of low-temperature plasma jets on tumor cell proliferation Влияние низкотемпературных плазменных струй на пролиферацию опухолевых клеток Евтина А А Evtina A A anastasiya10152@gmail.com Булдаков М А Buldakov M A Нехорошев В О Nekhoroshev V O Ландль Н В Landl N V Чердынцева Н В Cherdyntseva N V Королев Ю Д Korolev Y D Национальный исследовательский Томский государственный университет; НИИ онкологии, Томский национальный исследовательский медицинский центр РАН ru National Research Tomsk State University; Cancer Research Institute, Tomsk National Research Medical Center Russian Academy of Sciences ru НИИ онкологии, Томский национальный исследовательский медицинский центр РАН ru Cancer Research Institute, Tomsk National Research Medical Center Russian Academy of Sciences ru Институт сильноточной электроники СО РАН ru Institute of High Current Electronics, Siberian Branch Russian Academy of Sciences ru Институт сильноточной электроники СО РАН ru Institute of High Current Electronics, Siberian Branch Russian Academy of Sciences ru Национальный исследовательский Томский государственный университет; НИИ онкологии, Томский национальный исследовательский медицинский центр РАН ru National Research Tomsk State University; Cancer Research Institute, Tomsk National Research Medical Center Russian Academy of Sciences ru Институт сильноточной электроники СО РАН ru Institute of High Current Electronics, Siberian Branch Russian Academy of Sciences ru 25 09 2021 25 09 2021 6 3 464 471 20 09 2021 20 09 2021 https://rusjbpc.ru/en/nauka/article/54675/view

Существующая литература демонстрирует наличие противоопухолевого действия низкотемпературной плазмы как in vitro на разных клеточных линиях, так и in vivo. Данное направление интенсивно изучается в последнее время, однако, исследования осложняются ввиду большого разнообразия объектов исследования и недостаточной изученности плазменных струй. В данной работе изучалось влияние низкотемпературной плазменной струи на пролиферацию опухолевых клеток HeLa как в различных режимах обработки клеточной культуры, так и при вариации параметров источника плазмы. Низкотемпературные плазменные струи были получены на основе импульсного электрического разряда, поддерживаемого в потоке воздуха при атмосферном давлении. С целью поиска оптимальных условий варьировались частота следования импульсов напряжения от 0,5 до 4,2 кГц, время экспозиции от 30 до 240 с, количество повторов обработки и длительность паузы между повторами (10 минут, 24 часа, 72 часа). Было показано, что данный тип физического воздействия способен подавлять рост опухолевых клеток до 85% по сравнению с группой контроля. Данный эффект зависит как от частоты следования импульсов тока разряда, так и от времени воздействия. Полученные данные свидетельствуют о перспективности использования низкотемпературных плазменных струй для подавления пролиферации опухолевых клеток.

The existing literature demonstrates the presence of antitumor effect of low-temperature plasma both in vitro on different cell lines and in vivo. This area has been intensively studied recently, however, research is complicated due to the large variety of research objects and insufficient knowledge of plasma jets. In this paper, the effect of a low-temperature plasma jet on the proliferation of HeLa tumor cells was studied. Results of experiments in different modes of cell culture processing and with variations in the parameters of the plasma source were presented. Low-temperature plasma jets were obtained using of a pulsed electric discharge sustained in the air flow at atmospheric pressure. In order to find optimal conditions, the repetition frequency of voltage pulses from 0.5 to 4.2 kHz, the exposure time from 30 to 240 s, the number of processing repetitions and the duration of the pause between repetitions (10 minutes, 24 hours, 72 hours) were varied. It was shown that this type of physical impact is able to suppress the growth of tumor cells up to 85% compared to the control group. This effect depends both on the repetition frequency of the discharge current pulses and on the time of exposure. The obtained data show the prospects of using low-temperature plasma jets to suppress the tumor cells proliferation.

 низкотемпературная плазма разряд в потоке газа плазменная струя онкология пролиферация HeLa low-temperature plasma discharge in a gas stream plasma jet oncology proliferation HeLa

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