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 <front>
  <journal-meta>
   <journal-id journal-id-type="publisher-id">Russian Journal of Biological Physics and Chemisrty</journal-id>
   <journal-title-group>
    <journal-title xml:lang="en">Russian Journal of Biological Physics and Chemisrty</journal-title>
    <trans-title-group xml:lang="ru">
     <trans-title>АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ</trans-title>
    </trans-title-group>
   </journal-title-group>
   <issn publication-format="print">2499-9962</issn>
  </journal-meta>
  <article-meta>
   <article-id pub-id-type="publisher-id">54690</article-id>
   <article-categories>
    <subj-group subj-group-type="toc-heading" xml:lang="ru">
     <subject>Общая биофизика</subject>
    </subj-group>
    <subj-group subj-group-type="toc-heading" xml:lang="en">
     <subject>General biophysics</subject>
    </subj-group>
    <subj-group>
     <subject>Общая биофизика</subject>
    </subj-group>
   </article-categories>
   <title-group>
    <article-title xml:lang="en">Sodium bicarbonate as a carbon source for intensive cultivation of Porphyridium Purpureum (Bory) Drew et ross. on an industrial scale</article-title>
    <trans-title-group xml:lang="ru">
     <trans-title>Гидрокарбонат натрия как источник углерода для интенсивного культивирования Porphyridium Purpureum (Bory) Drew et Ross в промышленных масштабах</trans-title>
    </trans-title-group>
   </title-group>
   <contrib-group content-type="authors">
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Геворгиз</surname>
       <given-names>Р Г</given-names>
      </name>
      <name xml:lang="en">
       <surname>Gevorgiz</surname>
       <given-names>R G</given-names>
      </name>
     </name-alternatives>
     <email>r.gevorgiz@yandex.ru</email>
     <xref ref-type="aff" rid="aff-1"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Железнова</surname>
       <given-names>С Н</given-names>
      </name>
      <name xml:lang="en">
       <surname>Zheleznova</surname>
       <given-names>S N</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-2"/>
    </contrib>
    <contrib contrib-type="author">
     <name-alternatives>
      <name xml:lang="ru">
       <surname>Уваров</surname>
       <given-names>И П</given-names>
      </name>
      <name xml:lang="en">
       <surname>Uvarov</surname>
       <given-names>I P</given-names>
      </name>
     </name-alternatives>
     <xref ref-type="aff" rid="aff-3"/>
    </contrib>
   </contrib-group>
   <aff-alternatives id="aff-1">
    <aff>
     <institution xml:lang="ru">ФИЦ «Институт биологии южных морей имени А.О. Ковалевского РАН»</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Federal Research Center A.O. Kovalevsky Institute of Biology of the Southern Seas of the RAS</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-2">
    <aff>
     <institution xml:lang="ru">ФИЦ «Институт биологии южных морей имени А.О. Ковалевского РАН»</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Federal Research Center A.O. Kovalevsky Institute of Biology of the Southern Seas of the RAS</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <aff-alternatives id="aff-3">
    <aff>
     <institution xml:lang="ru">Управление ветеринарии города Новосибирска</institution>
     <country>ru</country>
    </aff>
    <aff>
     <institution xml:lang="en">Department of Veterinary Medicine of the city of Novosibirsk</institution>
     <country>ru</country>
    </aff>
   </aff-alternatives>
   <pub-date publication-format="print" date-type="pub" iso-8601-date="2021-12-25T20:22:29+03:00">
    <day>25</day>
    <month>12</month>
    <year>2021</year>
   </pub-date>
   <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2021-12-25T20:22:29+03:00">
    <day>25</day>
    <month>12</month>
    <year>2021</year>
   </pub-date>
   <volume>6</volume>
   <issue>4</issue>
   <fpage>554</fpage>
   <lpage>558</lpage>
   <history>
    <date date-type="received" iso-8601-date="2021-12-20T20:22:29+03:00">
     <day>20</day>
     <month>12</month>
     <year>2021</year>
    </date>
    <date date-type="accepted" iso-8601-date="2021-12-20T20:22:29+03:00">
     <day>20</day>
     <month>12</month>
     <year>2021</year>
    </date>
   </history>
   <self-uri xlink:href="https://rusjbpc.ru/en/nauka/article/54690/view">https://rusjbpc.ru/en/nauka/article/54690/view</self-uri>
   <abstract xml:lang="ru">
    <p>В работе представлены экспериментальные доказательства использования бикарбоната натрия в качестве единственного источника углерода при интенсивном культивирования Porphyridium purpureum. Показано, что питательные среды для интенсивного культивирования P. purpureum на основе морской воды могут содержать до 20 г·л-1 и более бикарбоната натрия в качестве источника углерода. При использовании питательной среды с бикарбонатом натрия максимальная плотность культуры достигала 3 г.с.б.·л-1 и более. Максимальная продуктивность в лабораторных фотобиореакторах достигала 1,16 г·л-1· сут-1. При культивировании P. purpureum в промышленных масштабах при естественном освещении продуктивность культуры зависела от световых условий. В пасмурную погоду, когда максимальная освещенность в течение суток составляла 7-12 клк, продуктивность достигала 6,33 г.с.б.·м-2 · сут-1. В то время как в солнечную погоду (максимальная освещённость рабочей поверхности в течение дня 50 клк) продуктивность составила 17,7 г.с.б.·м-2·сут-1. Отмечено, что при высокой облучённости у накопительных кривых не наблюдалось стационарной фазы роста. При достижении максимальной плотности культура переходила в фазу отмирания. Сделано предположение о том, что это связано с высокими значениями рН, при которых многие биогенные элементы становятся недоступными для клеток в связи с малой растворимостью некоторых солей в щелочной среде, а также переходу всех форм неорганического углерода в карбонатную форму.</p>
   </abstract>
   <trans-abstract xml:lang="en">
    <p>The paper presents experimental evidence for the use of sodium bicarbonate as the only carbon source during intensive cultivation of Porphyridium purpureum . It has been shown that culture media for intensive cultivation of P. purpureum based on sea water can contain up to 21 g / l of sodium bicarbonate as a carbon source. When using a nutrient medium with sodium bicarbonate, the maximum density of the culture reached 3 g.b./l and more. The maximum productivity in laboratory photobioreactors reached 1.16 g / (l · day). When P. purpureum was cultivated on an industrial scale under natural light, the productivity of the culture depended on light conditions. In cloudy weather, when the maximum illumination during the day was 7-12 klx, the productivity reached 6.33 g.d.b./ (m2 day). While in sunny weather (the maximum illumination of the working surface during the day is 50 klx), the productivity was 17.7 g.b./ (m2 day). It was noted that at high irradiation, the accumulation curves did not show a stationary growth phase. Upon reaching the maximum density, the culture passed into the phase of withering away. It has been suggested that this is due to high pH values, at which many biogenic elements become inaccessible to cells due to the low solubility of some salts in an alkaline medium, as well as the transition of all forms of inorganic carbon to the carbonate form.</p>
   </trans-abstract>
   <kwd-group xml:lang="ru">
    <kwd>низшие фототрофы</kwd>
    <kwd>питательная среда</kwd>
    <kwd>красные микроводоросли</kwd>
    <kwd>углерод-концентрирующий механизм</kwd>
    <kwd>пищевая сода</kwd>
   </kwd-group>
   <kwd-group xml:lang="en">
    <kwd>lower phototrophs</kwd>
    <kwd>nutrient medium</kwd>
    <kwd>red microalgae</kwd>
    <kwd>carbon-concentrating mechanism</kwd>
    <kwd>baking soda</kwd>
   </kwd-group>
   <funding-group>
    <funding-statement xml:lang="ru">Работа выполнена в рамках госзадания по теме НИР ФИЦ ИнБЮМ № 121030300149-0.</funding-statement>
   </funding-group>
  </article-meta>
 </front>
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