Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54702 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Mechanisms of lithium action on human erythrocytes Механизмы действия лития на эритроциты человека Слобожанина Е И Slobozhanina E I slobozhanina@ibp.org.by Зубрицкая Г П Zubritskaya G P Скоробогатова А С Skarabahatava A S Белевич Е И Belevich E I Венская Е И Venskaya E I Лукьяненко Л М Lukyanenko L M Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of NAS of Belarus ru 25 12 2021 25 12 2021 6 4 616 623 20 12 2021 20 12 2021 https://rusjbpc.ru/en/nauka/article/54702/view

Выявлено достоверное увеличение внутриклеточной концентрации лития после воздействия на эритроциты 6-10 мМ Li2SO4 в течение 3 ч. Накопление ионов лития в эритроцитах сопровождается ингибированием их эстеразной активности, незначительным снижением уровня активных форм кислорода и увеличением проницаемости клеточной мембраны. Показано, что воздействие солей лития в токсичных концентрациях на эритроциты человека in vitro незначительно снижает уровень активных форм кислорода, но приводит к модификации физико-химического состояния мембраносвязанных белков и липидов. Полученные результаты являются основой для создания клеточной тест-системы для оценки токсичности лития.

A significant increase in the intracellular concentration of lithium was revealed after exposure of erythrocytes to 6-10 mM Li2SO4 for 3 hours. The accumulation of lithium ions in erythrocytes is accompanied by inhibition of their esterase activity, a slight decrease in the level of reactive oxygen species and an increase in the permeability of the cell membrane. It has been shown that the effect of lithium salts in toxic concentrations on human erythrocytes in vitro slightly reduces the level of reactive oxygen species, but leads to a modification of the physicochemical state of membrane-bound proteins and lipids. The results obtained are the basis for creating a cell test system for assessing the toxicity of lithium.

 эритроциты литий микроэлементы активные формы кислорода микровязкость липидов мембраносвязанные белки erythrocytes lithium trace elements reactive oxygen species lipid microviscosity membrane-bound proteins

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