Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54812 10.29039/rusjbpc.2022.0476 Общая биофизика General biophysics Общая биофизика EFFECTS OF CARDIAC MYOSIN-BINDING PROTEIN C ON THE CHARACTERISTICS OF A SINGLE ACTIN-MYOSIN INTERACTION IN THE MYOCARDIUM Влияние сердечного миозин-связывающего белка С на характеристики одиночного актин-миозинового взаимодействия в миокарде Набиев С. Р. Nabiev S. R. salavatik2003@gmail.com Копылова Г. В. Kopylova G. V. Щепкин Д. В. Shchepkin D. V. Институт иммунологии и физиологии УрО РАН Екатеринбург Россия Institute of Immunology and Physiology of the UB RAS Yekaterinburg Russian Federation Институт иммунологии и физиологии УрО РАН Екатеринбург Россия Institute of Immunology and Physiology of the UB RAS Yekaterinburg Russian Federation Институт иммунологии и физиологии УрО РАН Екатеринбург Россия Institute of Immunology and Physiology of the UB RAS Yekaterinburg Russian Federation 25 03 2022 25 03 2022 7 1 13 16 20 03 2022 20 03 2022 https://rusjbpc.ru/en/nauka/article/54812/view

Сокращение миокарда является результатом взаимодействия миозина, составляющего толстую нить, с актином, образующим основу тонкой нити, и регулируется кальцием через регуляторные белки тропонин и тропомиозин. Недавно было установлено, что в регуляции актин-миозинового взаимодействия, кроме регуляторных белков, принимает участие сердечный миозин-связывающий белок-C (сMyBP-C). сMyBP-C является одним из интегральных белков саркомера кардиомиоцита, который имеет сайты связывания с основными белками саркомера, миозином, актином и тропомиозином. сMyBP-C контролирует количество миозиновых головок, взаимодействующих с тонкой нитью, и участвует в её активации. В работе исследовано влияние сMyBP-C на характеристики одиночного актин-миозинового взаимодействия, размер шага миозина и продолжительность взаимодействия, используя метод оптической ловушки. Сердечный миозин экстрагировали из миокарда левого желудочка кролика, актин выделяли из быстрых скелетных мышц кролика, а сMyBP-C получали из миокарда левого желудочка курицы. cMyBP-C добавляли к сердечному миозину в физиологическом соотношении 1:5. В in vitro подвижной системе обнаружено, что добавление cMyBP-C замедляет скольжение актина по миозину на 30%. С помощью оптической ловушки показано, что cMyBP-C не влияет на величину рабочего шага миозина, но увеличивает продолжительность его взаимодействия с актиновой нитью. Полученные результаты говорят о прямом влиянии cMyBP-C на одиночное актин-миозиновое взаимодействие.

. Myocardial contraction is the result of the interaction of myosin, which makes up the thick filament, with actin, which forms the basis of the thin filament, and is regulated by calcium through the regulatory proteins troponin and tropomyosin. Recently, it was found that, in addition to regulatory proteins, cardiac myosin-binding protein-C (cMyBP-C) is involved in the regulation of actin-myosin interaction. cMyBP-C is one of the integral proteins of the cardiomyocyte sarcomere, which has binding sites for the main sarcomere proteins, myosin, actin, and tropomyosin. cMyBP-C controls the number of myosin heads interacting with the thin filament and participates in its activation. In this work, the influence of cMyBP-C on the characteristics of a single actin-myosin interaction, myosin step size and interaction duration, was studied using an optical trap method. Cardiac myosin was extracted from rabbit left ventricular myocardium, actin was isolated from rabbit fast skeletal muscle, and cMyBP-C was obtained from chicken ventricles. cMyBP-C was added to cardiac myosin in a physiological ratio of 1:5. In an in vitro motility assay, the addition of cMyBP-C was found to slow actin sliding velocity over myosin by 30%. It was found that cMyBP-C does not affect step size of myosin but increases the duration of its interaction with the actin filament. The results obtained indicate a direct effect of cMyBP-C on a single actin-myosin interaction.

 сердечный миозин-связывающий белок С актин-миозиновое взаимодействие миокард оптическая ловушка cardiac myosin-binding protein C actin-myosin interaction myocardium optical trap Работа поддержана РНФ (грант №22-14-00174) и выполнена на оборудовании ЦКП ИИФ УрО РАН.

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