Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55010 10.29039/rusjbpc.2022.0510 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ INTERACTION OF METHYLENE BLUE WITH SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU) AND WITH DOUBLE_STRANDED POLYNUCLEOTIDE POLY(rA)-POLY(rU) Взаимодействие метиленового синего с одноцепочечными полинуклеотидами poly(rA) и poly(rU) и с двухцепочечным полинуклеотидом poly(rA)-poly(rU) Антонян А. П. Antonyan A. P. Мовсесян З. О. Movsesyan Z. O. Парсаданян М. А. Parsadanyan M. A. Вардеванян П. О. Vardevanyan P. O. p.vardevanyan@ysu.am Ереванский государственный университет Ереван Россия Yerevan State University Yerevan Russian Federation Ереванский государственный университет Ереван Армения Yerevan State University Yerevan Armenia Ереванский государственный университет Ереван Россия Yerevan State University Yerevan Russian Federation Ереванский государственный университет Ереван Армения Yerevan State University Yerevan Armenia 25 06 2022 25 06 2022 7 2 251 255 20 06 2022 20 06 2022 https://rusjbpc.ru/en/nauka/article/55010/view

Исследовано взаимодействие красителя-интеркалятора метиленового синего (МС) с одноцепочечными (оц-) poly(rA), poly(rU) и двухцепочечным poly(rA)-poly(rU) полинуклеотидами методами абсорбционной спектроскопии, при различных концентрационных соотношениях лиганд/фосфат (концентрация оц-полинуклеотидов по фосфатному остатку). Обнаружено, что в полосе поглощения метиленового синего, в интервале изменения длины волны 500≤λ≤750 нм спектры поглощения комплексов МС-оц-poly(rA) резко уменьшаются, по сравнению со спектром МС, по мере возрастания концентрации полинуклеотида в растворе. Также обнаружено, что спектры поглощения комплексов МС-оц-poly(rU) уменьшаются умеренно, при увеличении концентрации этого полинуклеотида в растворе. На спектрах комплексов МС-оц-poly(rU) образуется псевдоизобестическая точка, в то время как на спектрах комплексов МС-оц-poly(rA) такая точка не образуется. Псевдоизобестическая точка образуется и на спектрах комплексов МС-poly(rA)-poly(rU). Также обнаружено, что спектры поглощения комплексов МС с оц-poly(rA) и poly(rA)-poly(rU) сдвигаются в сторону длинных волн на ~5-7 нм, в то время как сдвиг спектров поглощения комплексов МС-оц-poly(rU) составляет всего ~2 нм. Изменения спектров поглощения комплексов МС с указанными полинуклеотидами, указывают на то, что МС проявляет высокую специфичность к poly(rA), по сравнению с poly(rU) и poly(rA)-poly(rU). Полученные данные также указывают на то, что МС с poly(rA) и poly(rA)-poly(rU) связывается интеркаляционным и электростатическим, а с оц-poly(rU) – в основном электростатическим механизмами.

Interaction of dye-intercalator methylene blue (MB) with single-stranded (ss-) polynucleotides poly(rA), poly(rU) and double-stranded poly(rA)-poly(rU) has been studied by the method of absorption spectroscopy at various concentration ratios ligand/phosphate (concentration of ss-polynucleotides by phosphate residue). It was revealed that in the absorption layer of methylene blue, in the wavelength change interval 500≤λ≤750 nm, the absorption spectra of the complexes MB-ss-poly(rA) decrease more sharply, as compared to MB spectrum, along with polynucleotide concentration enhancement in the solution. It was also revealed that the absorption spectra of the complexes MB-ss-poly(rU) decrease moderately at the increase of this polynucleotide concentration in the solution. In the spectra of the complexes MB-ss-poly(rU) a pseudo-isosbestic point is formed, while in the spectra of the complexes MB-ss-poly(rA) there is no such point. The pseudo-isosbestic point is formed also in the spectra of the complexes MB-poly(rA)-poly(rU). It was also revealed that the absorption spectra of the complexes of MB with ss-poly(rA) and poly(rA)-poly(rU) are shifted to the longer wavelengths by ~5-7 nm, while the shift in the absorption spectra of the complexes MB-ss-poly(rU) composes almost ~2 nm. The absorption spectra changes of the complexes of MB with the mentioned polynucleotides indicate that MB shows higher specificity to poly(rA), as compared to poly(rU) and poly(rA)-poly(rU). The obtained data also indicate that MB binds to poly(rA) and poly(rA)-poly(rU) by intercalation and electrostatic modes, and with ss-poly(rU) – mainly by electrostatic mechanism.

 метиленовый синий полуинтеркаляционный механизм poly(rA) poly(rU) poly(rA)-poly(rU) электростатическое связывание спектры поглощения methylene blue semi-intercalation mechanism poly(rA) poly(rU) poly(rA)-poly(rU) electrostatic binding absorption spectra Исследование выполнено при финансовой поддержке Комитета по науке РА в рамках научного проекта № 21T-1F063.

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