Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55012 10.29039/rusjbpc.2022.0512 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ BENZOATE GROUP ATTACHMENT TO TEMPO PROVIDES ENHANCED DISCRIMINATION OF LIPOSOMES FABRICATED USING HUMAN LUNG NORMAL AND CARCINOMA CELLS Benzoate group attachment to TEMPO provides enhanced discrimination of liposomes fabricated using human lung normal and carcinoma cells Касумов О. К. Gasymov O. K. ogassymo@g.ucla.edu

доктор физико-математических наук;

doctor of physical and mathematical sciences;

 Бахышова М. Д. Bakhishova M. J. Гасанова Р. Б. Gasanova R. B. Асланов Р. Б. Aslanov R. B. Меликова Л. А. Melikova L. A. Алиев Д. А. Aliyev J. A. Институт биофизики НАНА Баку Россия Institute of Biophysics of Azerbaijan National Academy of Sciences Baku Russian Federation Институт биофизики НАНА Баку Россия Institute of Biophysics of Azerbaijan National Academy of Sciences Baku Russian Federation Институт биофизики НАНА Баку Россия Institute of Biophysics of Azerbaijan National Academy of Sciences Baku Russian Federation Институт биофизики НАНА Баку Россия Institute of Biophysics of Azerbaijan National Academy of Sciences Baku Russian Federation Институт биофизики НАНА Баку Россия Institute of Biophysics of Azerbaijan National Academy of Sciences Baku Russian Federation Национальный центр онкологии Министерства здравоохранения Азербайджанской Республики Баку Россия National Center of Oncology, Azerbaijan Republic Ministry of Health Baku Russian Federation Национальный центр онкологии Министерства здравоохранения Азербайджанской Республики Баку Россия National Center of Oncology, Azerbaijan Republic Ministry of Health Baku Russian Federation 25 06 2022 25 06 2022 7 2 261 267 20 06 2022 20 06 2022 https://rusjbpc.ru/en/nauka/article/55012/view

It is widely accepted that the lipid compositions of the plasma membranes of healthy and cancer cells significantly differ from each other. During the cancer progression, cancer cells change the lipid constituent of the membranes resulting in the loss of lipid asymmetry between the membrane leaflets. Consequently, physicochemical properties of the cell membranes are also changed in response to altered lipid organization. Partitioning of the spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) into the membranes of the cells has broadly been applied to characterize membrane properties of various cells in health and disease conditions. In this work, we used liposomes fabricated using lipids extracted from normal and carcinoma cells. This system permits the determination of the properties of the healthy and cancer cell membranes provided exclusively by its lipid components. Application of TEMPO-benzoate, in which the benzoate group is attached to the TEMPO, indicates significantly enhanced discrimination of liposomes between cancer and normal cells. Partitioning experiments with TEMPO-benzoate revealed relatively enhanced incorporation efficiency for liposomes of cancer cells. On the contrary, TEMPO incorporation efficiency in the same liposomes of cancer cells was not much different compared to healthy cells. Data indicate that TEMPO-benzoate as a probe is more suitable than TEMPO to discriminate cancer cells from healthy cells. Free energy gain observed for TEMPO-benzoate resulted mainly from the hydrophobic effect of the benzoate group.

It is widely accepted that the lipid compositions of the plasma membranes of healthy and cancer cells significantly differ from each other. During the cancer progression, cancer cells change the lipid constituent of the membranes resulting in the loss of lipid asymmetry between the membrane leaflets. Consequently, physicochemical properties of the cell membranes are also changed in response to altered lipid organization. Partitioning of the spin probe 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) into the membranes of the cells has broadly been applied to characterize membrane properties of various cells in health and disease conditions. In this work, we used liposomes fabricated using lipids extracted from normal and carcinoma cells. This system permits the determination of the properties of the healthy and cancer cell membranes provided exclusively by its lipid components. Application of TEMPO-benzoate, in which the benzoate group is attached to the TEMPO, indicates significantly enhanced discrimination of liposomes between cancer and normal cells. Partitioning experiments with TEMPO-benzoate revealed relatively enhanced incorporation efficiency for liposomes of cancer cells. On the contrary, TEMPO incorporation efficiency in the same liposomes of cancer cells was not much different compared to healthy cells. Data indicate that TEMPO-benzoate as a probe is more suitable than TEMPO to discriminate cancer cells from healthy cells. Free energy gain observed for TEMPO-benzoate resulted mainly from the hydrophobic effect of the benzoate group.

 Electron paramagnetic resonance TEMPO TEMPO-benzoate partitioning lung carcinoma cell membrane lipid composition Electron paramagnetic resonance TEMPO TEMPO-benzoate partitioning lung carcinoma cell membrane lipid composition This work was supported by the Science Development Foundation under the President of the Republic of Azerbaijan- Grant № EİF-MQM-ETS-2020-1(35)-08/07/3-M-07.

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