Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55018 10.29039/rusjbpc.2022.0518 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ IN THE ERYTHROCYTE HEMOGLOBIN CONFORMATION AND DISTRIBUTION DURING RBC VOLUME CHANGES Изменение конформации и распределения гемоглобина при изменении объема эритроцита Слатинская О. В. Slatinskaya O. V. slatolya@mail.ru Максимов Г. В. Maksimov G. V. Московский государственный университет им. М.В. Ломоносова Москва Россия Lomonosov Moscow State University Moscow Russian Federation Московский государственный университет им. М.В. Ломоносова Москва Россия Lomonosov Moscow State University Moscow Russian Federation 25 06 2022 25 06 2022 7 2 297 302 20 06 2022 20 06 2022 https://rusjbpc.ru/en/nauka/article/55018/view

Эритроцит представляет собой клетку, внутриклеточное пространство которой на 40% состоит из молекул гемоглобина (Гб), остальные 60% приходятся на молекулы воды. При изменении внутриклеточного объема, которое возможно при прохождении клеток эритроцита по кровеносному руслу, предполагается возможность перераспределения внутриклеточных компонентов клетки вследствие изменения её объема. Методами оптической спектроскопии (неинвазивные методы инфракрасной спектроскопии, Раман-спектроскопии, лазерной интерференционной микроскопии) выявлены изменения морфологии, конформации и перераспределения молекул Гб в эритроците человека в следствие увеличения соотношения [Na+]in и [K+]in при блокировании Na+/K+-АТФазы клетке. Установлено, что снижение активности работы Na+/K+-АТФазы при помощи блокатора уабаина (3 мМ), приводит не только к увеличению [Na+]in в клетке, но и увеличению положительного заряда на цитоплазматической поверхности плазматической мембраны эритроцита. В этих условиях выявлены изменения конформации как гемовой, так и глобиновой части цитоплазматического Гб. Показано, что деполяризация клетки, вместе с изменением объема клетки, приводит к снижению плотности упаковки молекулы Гб, которые могут быть связаны как с сорбцией внутриклеточного Na+ (или Са2+) с Гб, так и увеличением количества молекул воды в клетке и перераспределением Гб в клетке. Данные процессы могут приводить к изменению конформации примембранного Гб, так и к перераспределению и изменению конформации цитоплазматического Гб.

The intracellular space of red blood cells (RBC) includes 40% hemoglobin (Hb) molecules and 60% being water molecules. When the intracellular volume changes, which is possible when erythrocyte cells pass through the blood stream, the possibility of redistribution of intracellular components of the cell due to its volume changes is assumed. By methods of optical spectroscopy (non-invasive methods of infrared spectroscopy, Raman-spectroscopy, laser interference microscopy) changes of morphology, conformation and redistribution of Hb have been revealed in the human erythrocyte due to an increase in the ratio of [Na+]in and [K+]in when Na+/K+-ATPase is blocked in the cell. The decrease of activity of Na+/K+-ATPase by a ouabaine (3 mM) has been found to lead not only to an increase in [Na+]in cell but also to an increase in positive charge on the cytoplasmic surface of the RBC membrane. In these conditions, changes in the conformation of both the heme and globine parts of the cytoplasmic Hb have been identified. It has been shown that cell depolarization, together with cell volume change, leads to a decrease in packing density of Hb molecule, which may be related to sorption of intracellular Na+ (or Ca2+) c Hb, as well as increasing the amount of water molecules in the cell and redistributin Hb in the cell. These processes can lead to a change in the conformation of Hb, as well as to a redistribution and alteration of the conformation of the cytoplasmic Hb.

 гемоглобин конформация уабаин Раман-спектроскопия инфракрасная спектроскопия hemoglobin conformation Raman-spectroscopy ouabain Исследование выполнено при финансовой РФФИ (№ 20-34-90073, для С.О.В.)

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