Sugar beet, a biennial industrial agricultural crop, is used for the production of ethanol, biofuels, sugar and contains up to 70% sucrose. It is difficult to obtain seeds, the collection of which takes place in the second year of cultivation of root crops. The possibility of accelerating seed activation, increasing germination by methods of pre-sowing treatment of dry seeds: laser pulsed radiation and ecoperoxide solutions was studied. The first test indicating the beginning of seed awakening (one hour after treatment) was a test for determining the release of metabolites into the aqueous culture medium. The metabolites determined by spectrophotometry. They have a characteristic absorption spectrum in the UV region with a clear maximum at λmax – 207 nm and absorption up to the boundary with the visible region of λmax – 330 nm. A copper vapor laser was used. Parameters: simultaneous generation of two wavelengths – green 510.5 nm and yellow 578.2 nm. The duration of the radiation pulse is 15 ns, the repetition rate is 10 kHz. The irradiation time is 10, 20, 90 s. The greatest effect of seed germination stimulation at 10 s of irradiation exceeded the control by 30%. Increasing the interval to 24 hours or more between irradiation and placing seeds for germination reduced the number of germinated seeds. Ecoperoxide was obtained by high-energy non-contact exposure to distilled water with high-voltage electric discharge streamers. An increase in seed germination by 30-40% was noted when they came into contact with a peroxide solution for 30 minutes.