Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55119 10.29039/rusjbpc.2022.0541 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ WAYS OF REALIZATION OF APOPTOSIS OF HUMAN LYMPHOCYTES INDUCED BY HYDROGEN PEROXIDE AND THEIR REGULATION ПУТИ РЕАЛИЗАЦИИ АПОПТОЗА ЛИМФОЦИТОВ ЧЕЛОВЕКА, ИНДУЦИРОВАННОГО ПЕРОКСИДОМ ВОДОРОДА, И ИХ РЕГУЛИРОВАНИЕ Наквасина М. А. Nakvasina M. A. nakvasina_ma@mail.ru Артюхов В. Г. Artyukhov V. G. Старикова Т. И. Starikova T. I. Сараджи Н. Г. Saradzhi N. G. Воронежский государственный университет Воронеж Россия Voronezh State University Voronezh Russian Federation Воронежский государственный университет Воронеж Россия Voronezh State University Voronezh Russian Federation Воронежский государственный университет Воронеж Россия Voronezh State University Voronezh Russian Federation Воронежский государственный университет Воронеж Россия Voronezh State University Voronezh Russian Federation 28 09 2022 28 09 2022 7 3 440 445 20 09 2022 20 09 2022 https://rusjbpc.ru/en/nauka/article/55119/view

В работе изучены изменения маркерных показателей апоптотической гибели клеток: уровня функциональной активности инициирующих каспаз-8, -9, -12, мембранных рецепторов смерти Fas, внутриклеточных активных форм кислорода, цитозольного кальция, митохондриального потенциала лимфоцитов периферической крови доноров, индуцированные воздействием пероксида водорода в конечных концентрациях 10-6 и 10-5 моль/л. Обнаружена активация инициирующей каспазы-8, ассоциированной с мембранными рецепторами смерти Fas, после воздействия на лимфоциты пероксида водорода в концентрации 10-6 моль/л. Выявлено значительное повышение функциональной активности инициирующей каспазы-9, уровня Fas-рецепторов и внутриклеточных активных форм кислорода, а также снижение концентрации цитозольного кальция и величины мембранного потенциала митохондрий через 1 и 2 ч после модификации клеток пероксидом водорода в концентрации 10-5 моль/л по сравнению с таковыми для интактных иммуноцитов. Установлено, что апоптоз лимфоцитов в условиях воздействия экзогенного пероксида водорода реализуется с участием рецепторопосредованного каспазного, митохондриального (с активацией каспазы-9) и р53-зависимого механизмов. Выявлено защитное действие ресвератрола (10-6 моль/л) на лимфоциты после воздействия пероксида водорода (10-5 моль/л), которое обусловлено его способностью снижать уровень активных форм кислорода в интактных и модифицированных пероксидом водорода иммуноцитах, а также дезактивировать пероксид водорода.

The changes in marker indicators of apoptotic cell death were studied: the level of functional activity of initiating caspases-8, -9, -12, membrane death receptors Fas, intracellular reactive oxygen species, cytosolic calcium, mitochondrial potential of peripheral blood lymphocytes of donors induced by exposure to hydrogen peroxide at final concentrations of 10-6 and 10-5 mol/l. Activation of initiating caspase-8 associated with membrane death receptors Fas was detected after exposure to hydrogen peroxide lymphocytes at a concentration of 10-6 mol/l. There was a significant increase in the functional activity of initiating caspase-9, the level of Fas receptors and intracellular reactive oxygen species, as well as a decrease in the concentration of cytosolic calcium and the value of the mitochondrial membrane potential 1 and 2 hours after modification of cells with hydrogen peroxide at a concentration of 10-5 mol/l compared with those for intact immunocytes. It was found that apoptosis of lymphocytes under the influence of exogenous hydrogen peroxide is realized with the participation of receptor-mediated caspase, mitochondrial (with activation of caspase-9) and p53-dependent mechanisms. The protective effect of resveratrol (10-6 mol/l) on lymphocytes after exposure to hydrogen peroxide (10-5 mol / l), which is due to its ability to reduce the level of reactive oxygen species in intact and modified hydrogen peroxide immunocytes, as well as deactivate hydrogen peroxide.

 апоптоз лимфоциты пероксид водорода каспазы apoptosis lymphocytes hydrogen peroxide caspases Работа выполнена при поддержке Министерства науки и высшего образования РФ в рамках государственного задания ВУЗам в сфере научной деятельности на 2020—2022 годы, проект № FZGU-2020-0044.

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