Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55128 10.29039/rusjbpc.2022.0550 ЭКОЛОГИЧЕСКАЯ БИОФИЗИКА ECOLOGICAL BIOPHYSICS ЭКОЛОГИЧЕСКАЯ БИОФИЗИКА CHARACTERISTICS OF THE CATIONIC TRPA1-CHANNALS FAMILY IN TRICHOPLAX SP. H2 (PLACOZOA) ХАРАКТЕРИСТИКА СЕМЕЙСТВА КАТИОННЫХ TRPА1-КАНАЛОВ TRICHOPLAX SP. H2 (PLACOZOA) Фадеева М. В. Fadeeva M. V. Сергеева Е. В. Sergeeva E. V. Рыбакова К. А. Rybakova K. A. Кузнецов А. В. Kuznetsov A. V. kuznet61@gmail.com Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Центр дополнительного образования «Малая академия наук» Севастополь Россия Center for Additional Education "Small Academy of Sciences" Sevastopol Russian Federation Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of the RAS Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation 28 09 2022 28 09 2022 7 3 493 500 20 09 2022 20 09 2022 https://rusjbpc.ru/en/nauka/article/55128/view

Механорецепторы способны преобразовывать силовые воздействия в электрохимические сигналы, которые инициируют волну деполяризации, распространяющуюся по поверхности клетки. В Drosophila melanogaster известен канал механотрансдукции TRPN1/NompC в составе которого находятся анкириновые повторы, выполняющие роль стробирующей спирали [1]. В этой работе мы впервые представляем семейство катионных TRPА1-каналов у Trichoplax sp. H2 (Placozoa), состоящее из 42 гомологов длиной от 468 до 3240 aa. Внутри семейства выделено три обособленных кластера с разной длиной полипептидной цепи и различным содержанием анкириновых повторов. Реконструирована пространственная структура TRPА1-канала RDD36842.1, проведён докинг аспирина – возможного блокатора TRP-каналов. Анализируется структура членов семейства, рассматривается механическая модель работы канала, а также обсуждается вероятная функция и физиология этих белковых молекул и их роль в поведении трихоплакса. Вариации числа анкириновых повторов на N-конце исследованных TRP-протеинов и обнаруженные различия на C-конце могут свидетельствовать о полифункциональности данных белков у Placozoa. Возможно, TRP-каналы возникли в ходе ранней эволюции до появления многоклеточности, но диверсифицировали в многоклеточных животных с усложнением структурной организации последних.

Mechanoreceptors can transform external mechanical forces into electrochemical signals that induce cell membrane depolarization and initiate waves propagating along the cell surface. The TRPN1/NompC mechanotransduction channel is known to contain ankyrin repeats that act as a gating helix in Drosophila melanogaster [Jin et al., 2017]. In this work, we present for the first time a family of cationic TRPA1-channels in Trichoplax sp. H2 (Placozoa), which consists of 42 homologs ranging from 468 to 3240 aa in length. Three isolated clusters with different polypeptide chain lengths and various contents of ankyrin repeats were identified within the family. The spatial structure of TRPA1-channel RDD36842.1 was reconstructed, and aspirin, a possible TRP-channel blocker, was docked. The structure of the family members is analyzed, the mechanical model of channel operation is considered, and the probable function and physiology of these protein molecules and their role in the behavior of trichoplax are discussed. Variations in the number of ankyrin repeats at the N-terminus of TRP-proteins studied, and the differences detected at the C-terminus can indicate the polyfunctionality of these proteins in Placozoa. Perhaps TRP channels arose in early evolutionary stages before the appearance of multicellularity, but diversified in multicellular animals as the structural organization of those became more complex.

 Placozoa механотрансдукция TRP-каналы пространственная реконструкция докинг Placozoa mechanotransduction TRP-channels protein 3D-reconstruction docking Работа выполнена в рамках государственного задания ФИЦ ИнБЮМ тема № 0828-2018-0002 и в ходе проекта Сириус «Трихоплакс для бионики II».

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