Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55184 10.29039/rusjbpc.2022.0578 ЭКОЛОГИЧЕСКАЯ БИОФИЗИКА ECOLOGICAL BIOPHYSICS ЭКОЛОГИЧЕСКАЯ БИОФИЗИКА RESPIRATORY PROPERTIES OF BLOOD PLANILIZA HAEMATOCHEILUS (TEMMINCK & SCHLEGEL, 1845) AND ZOSTERISESSOR OPHIOCEPHALUS (PALLAS, 1814) РЕСПИРАТОРНЫЕ СВОЙСТВА КРОВИ PLANILIZA HAEMATOCHEILUS (TEMMINCK & SCHLEGEL, 1845) И ZOSTERISESSOR OPHIOCEPHALUS (PALLAS, 1814) Солдатов Александр Александрович Soldatov Aleksandr Aleksandrovich alekssoldatov@yandex.ru

доктор биологических наук;

doctor of sciences in biology;

 Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of the RAS Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation 24 11 2022 24 11 2022 7 4 657 661 03 08 2022 https://rusjbpc.ru/en/nauka/article/55184/view

Естественная подвижность организма сказывается на функциональном состоянии и развитии многих систем органов и тканей. Она затрагивает, прежде всего, системы кислородного обеспечения: респираторную, циркуляционную, кровь. Особый интерес представляют молекулярные комплексы, функциональные характеристики детерминированы на генетическом уровне. К ним следует отнести респираторные пигменты, гемоглобины в частности, которым и посвящена настоящая работа. Исследовали газотранспортные свойства крови высокоподвижной кефали-пиленгаса (Planiliza haematocheilus) и донного малоподвижного вида – бычка-травяника (Zosterisessor ophiocephalus). Кровь получали пункцией хвостовой артерии. В качестве антикоагулянта применяли гепарин. Кровь пиленгаса имела более высокую концентрацию гемоглобина и число эритроцитов. Различия в отношении гемоглобина составляли почти 40 % (p <0,01), по эритроцитам около 2-х раз (p <0,01) (p <0,001). В отношении среднеклеточного содержания гемоглобина (МСН) результаты были противоположны. Кровь пиленгаса отличалась низким сродством к кислороду и повышенной чувствительностью к рН. Величина Р50 и значения эффекта Бора (r) у пиленгаса были на 25-26 % (p <0,01) и в 2,7-2,8 раз (p <0,001) соответственно выше, чем у травяника. Значения коэффициента Хилла (n), внутриклеточные концентрации нуклеотидтрифосфатов и Cl- совпадали у обоих видов. Полученные различия отражают процесс адаптации организма пиленгаса к высокоподвижному образу жизни.

The natural mobility of the organisms affects the functional state and development of many organ systems and tissues. It influences, first of all, oxygen supply systems: respiratory, circulatory, blood. Of particular interest are molecular complexes whose functional characteristics are determined at the genetic level. These include respiratory pigments, hemoglobin in particular, to which this work is devoted. The gas transport properties of the blood of a highly mobile mullet-pilengas (Planiliza haematocheilus) and a sedentary bottom species – grass goby (Zosterisessor ophiocephalus) were studied. Blood was obtained by puncture of the caudal artery. Heparin was used as an anticoagulant. Pilengas' blood had a higher concentration of hemoglobin and the number of red blood cells. The differences in hemoglobin were almost 40% (p <0.01), in erythrocytes about 2 times (p <0.01) (p <0.001). With respect to the mean cell hemoglobin content (MCN), the results were the opposite. Pilengas' blood was characterized by low affinity for oxygen and increased sensitivity to pH. The value of P50 and the values of the Born effect (r) in pilengas were 25-26% (p <0.01) and 2.7-2.8 times (p <0.001), respectively, higher than in grass goby. The values of the Hill coefficient (n), intracellular concentrations of nucleotide triphosphates and Cl- coincided in both species. The differences obtained reflect the process of adaptation of the pilengas organism to a highly mobile lifestyle.

 гемоглобин эритроциты кривые диссоциации крови внутриэритроцитарная среда Planiliza haematocheilus Zosterisessor ophiocephalus hemoglobin erythrocytes blood dissociation curves intra-erythrocyte medium Planiliza haematocheilus Zosterisessor ophiocephalus Работа выполнена в рамках госзадания (№ гос. регистрации АААА-А18-118021490093-4) и при частичной поддержке проекта РФФИ (№ 20-04-00037).

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