Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 83235 10.29039/rusjbpc.2023.0606 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ INTERACTION OF LIPOPOLYSACCHARIDE-BINDING PROTEINS WITH VARIOUS FORMS OF LIPOPOLYSACCHARIDES ВЗАИМОДЕЙСТВИЕ ЛИПОПОЛИСАХАРИД-СВЯЗЫВАЮЩИХ БЕЛКОВ С РАЗЛИЧНЫМИ ФОРМАМИ ЛИПОПОЛИСАХАРИДОВ Набережных Г. А. Naberezhnykh G. A. naber1953@mail.ru Давыдова В. Н. Davydova V. N. vikdavidova@yandex.ru Соловьева Т. Ф. Soloveva T. F. Тихоокеанский институт биоорганической химии им. Г.Б. Елякова ДВО РАН Владивосток Россия G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences Vladivostok Russian Federation Тихоокеанский институт биоорганической химии им. Г.Б. Елякова ДВО РАН Владивосток Россия G.B. Elyakov Pacific Institute of Bioorganic Chemistry FEB RAS Vladivostok Russian Federation Тихоокеанский институт биоорганической химии им. Г.Б. Елякова ДВО РАН Владивосток Россия G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences Vladivostok Russian Federation 21 05 2024 21 05 2024 8 2 178 184 14 07 2023 https://rusjbpc.ru/en/nauka/article/83235/view

Выделены и очищены липополисахарид-связывающие белки из двух массовых видов медуз Aurellia аurita и Ropelema asamushi и изучено взаимодействие липополисахаридов (ЛПС) разных структурных типов с ЛСБ. Ингибированием взаимодействия установлено, что оба белка специфически связываются с липидным и коровым фрагментами молекулы ЛПС. В ЛСБ присутствует два типа сайтов связывания с Кd = 3,28×10-6 М и Кd = 0,13×10-6 М (для белка из A. aurita) и Kd= 3,66 × 10-6 M и Kd = 0,27 × 10-6 M (для белка из R. asamushi). Методом динамического рассеяния света показано, что связывание ЛСБ с R-ЛПС приводит к диссоциации мицелл ЛПС. Размеры агрегатов ЛПС уменьшаются с 200 нм до 25-30 нм в составе комплексов ЛПС-ЛСБ. Данные электрокинетических измерений указывают на нейтрализацию отрицательного заряда R-ЛПС (-42,2 мВ) в комплексе ЛПС-ЛСБ- R. asamushi до -4,4 мВ. Мицеллы S-ЛПС из E. coli не дезагрегируют при связывании с ЛСБ, что, по-видимому, связано с экранированием липида А О-специфическими цепями в молекуле S-ЛПС. Связывание ЛПС с ЛСБ может оказывать влияние на их эндотоксические свойства.

Lipopolysaccharide-binding proteins from two common jellyfish species Aurellia aurita and Ropelema asamushi were isolated and purified, and the interaction of lipopolysaccharides (LPS) of various structural types with LBP was studied. By inhibiting the interaction, it was found that both proteins specifically bind to the lipid and core fragments of the LPS molecule. There are two types of binding sites in LBP with Kd = 3,28 × 10-6 M and Kd = 0,13 × 10-6 M (for the protein from A. aurita) and Kd = 3,66 × 10-6 M and Kd = 0,27 × 10-6 M (for protein from R. asamushi). It has been shown by dynamic light scattering that the binding of LBP to R-LPS leads to the dissociation of LPS micelles. The sizes of LPS aggregates decrease from 200 nm to 25–30 nm in the composition of LPS–LSB complexes. The data of electrokinetic measurements indicate the neutralization of the negative charge of Rd-LPS (-42,2 mV) in the LPS-LSB-R. asamushi complex up to -4,4 mV. S-LPS micelles from E. coli do not disaggregate upon binding to LBP, which is apparently due to the shielding of lipid A by O-specific chains in the S-LPS molecule. The binding of LPS to LBP may affect their endotoxic properties.

 липополисахаид липополисахарид связывающие белки параметры связывания дезагрегация lipopolysaccharide lipopolysaccharide binding proteins binding parameters disaggregation

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