Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 83370 10.29039/rusjbpc.2023.0627 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ INFLUENCE OF THE BLOOD FLOW THROUGH THE CAPILLARY ON THE MAIN PARAMETERS OF ERYTHROCYTES ВЛИЯНИЕ ПРОХОЖДЕНИЯ КРОВИ ЧЕРЕЗ КАПИЛЛЯР НА ОСНОВНЫЕ ПАРАМЕТРЫ ЭРИТРОЦИТОВ Ястребова Е. С. Yastrebova E. S. kat30cer@kinetics.nsc.ru Лагунов Т. А. Lagunov T. A. Институт химической кинетики и горения им. В.В. Воеводского Новосибирск Россия Voevodsky Institute of Chemical Kinetics and Combustion Novosibirsk Russian Federation Институт химической кинетики и горения им. В.В. Воеводского Новосибирск Россия Voevodsky Institute of Chemical Kinetics and Combustion Novosibirsk Russian Federation 27 05 2024 27 05 2024 8 3 319 327 19 07 2023 https://rusjbpc.ru/en/nauka/article/83370/view

Тренды персонализированной медицины приводят к необходимости определять норму биологических параметров для каждого отдельного человека. Такая задача требует большой точности получаемых параметров и достаточно частого их измерения. Для обеспечения высокой точности в определении морфологических и функциональных параметров клеток крови хорошо себя зарекомендовал метод сканирующей проточной цитометрии (СПЦ). В рамках данной работы идет разработка системы безыгольной венепункции для обеспечения более комфортных условий забора крови при частом отслеживании своих параметров. Однако, хотя в такой системе и возможно делать в коже отверстия значительно меньше, чем позволяет игла, возникает вопрос могут ли столь маленькие отверстия повлиять на параметры клеток крови, измеряемые на СПЦ. В работе выявлен первый из возможных влияющих на параметры клеток крови факторов – сдвиговое напряжение. Было исследовано поведение параметров эритроцитов при прохождении игл различного диаметра. Проведено моделирование в COMSOL распределения действующих сил на поверхности эллипсоида (как модели тромбоцита и эритроцита) в двух случаях: свободного перемещения клетки в капилляре и для прикрепленной к подложке клетке.

The trends in personalized medicine lead to the necessity of determining the normal range of biological parameters for each individual. This task requires high precision in the obtained parameters and frequent measurements. To ensure high accuracy in determining the morphological and functional parameters of blood cells, the method of scanning flow cytometry (SFC) has proven to be effective. Within this study, a needle-free venipuncture system is being developed to provide more comfortable conditions for blood collection during frequent monitoring of one's parameters. However, although such a system allows for much smaller skin punctures than a needle, the question arises as to whether these small openings can affect the blood cell parameters measured by SFC. This study identified the first potential factor influencing blood cell parameters - shear stress. The behavior of erythrocyte parameters was investigated as needles of different diameters passed through them. COMSOL modeling was conducted to analyze the distribution of forces acting on the surface of an ellipsoid (as a model for platelets and erythrocytes) in two scenarios: free cell movement in a capillary and for a cell attached to a substrate.

 эритроциты анионный обмен сдвиговое напряжение сканирующий проточный цитометр erythrocytes anion exchange shear stress scanning flow cytometer

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