Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 53985 Общая биофизика General biophysics Общая биофизика COMPENSATORY RESPONSE OF 2-CELL MOUSE EMBRYO TO HYPOOSMOTIC STRESS COMPENSATORY RESPONSE OF 2-CELL MOUSE EMBRYO TO HYPOOSMOTIC STRESS Pogorelov A G Pogorelov A G agpogorelov@rambler.ru Panait A I Panait A I Pogorelova V N Pogorelova V N Institute of Theoretical and Experimental Biophysics, RAS ru Institute of Theoretical and Experimental Biophysics, RAS ru Institute of Theoretical and Experimental Biophysics, RAS ru Institute of Theoretical and Experimental Biophysics, RAS ru Institute of Theoretical and Experimental Biophysics, RAS ru Institute of Theoretical and Experimental Biophysics, RAS ru 25 06 2016 25 06 2016 1 1 27 31 20 06 2016 20 06 2016 https://rusjbpc.ru/en/nauka/article/53985/view

Compensatory response of blastomeres in two-cell mouse embryo to hypoosmotic stress was studied employing the direct measurement of cellular volume with Laser Scanning Microtomography followed by 3-D reconstruction (QLSM). It was shown that embryos exposed to hypotonicity first swelled and then returned to the initial size. Swelling phase was defined by a water permeability coefficient (Lp) of 0.4 micron/(min.atm). The next compensatory phase of regulatory volume decrease (RVD) in embryonic cells was not dependent on Na+/K+-ATPase inhibition or low pH (6.4), but RVD was abolished by Cytochalasine B (Cyto B) treatment.

Compensatory response of blastomeres in two-cell mouse embryo to hypoosmotic stress was studied employing the direct measurement of cellular volume with Laser Scanning Microtomography followed by 3-D reconstruction (QLSM). It was shown that embryos exposed to hypotonicity first swelled and then returned to the initial size. Swelling phase was defined by a water permeability coefficient (Lp) of 0.4 micron/(min.atm). The next compensatory phase of regulatory volume decrease (RVD) in embryonic cells was not dependent on Na+/K+-ATPase inhibition or low pH (6.4), but RVD was abolished by Cytochalasine B (Cyto B) treatment.

 two-cell mouse embryo regulatory volume decrease Na+/K+-ATPase Cytochalasine B laser scanning microscopy 3D-reconstruction two-cell mouse embryo regulatory volume decrease Na+/K+-ATPase Cytochalasine B laser scanning microscopy 3D-reconstruction

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