Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54002 Общая биофизика General biophysics Общая биофизика INHIBITION OF PHOTOSYSTEM II ELECTRON-TRANSPORT CHAIN BY AMMONIA AND “DECOUPLING EFFECT” РАЗОБЩЕНИЕ ФУНКЦИИ ВЫДЕЛЕНИЯ КИСЛОРОДА И ЭЛЕКТРОННОГО ТРАНСПОРТА В ФОТОСИСТЕМЕ 2 АММОНИЕМ Ловягина Е Р Lovyagina E R Elena.Lovyagina@gmail.com Белевич Н П Belevich N P Сёмин Б К Semin B K ФГБОУ ВО «Московский государственный университет им. М.В. Ломоносова» ru Moscow State University ru ФГБОУ ВО «Московский государственный университет им. М.В. Ломоносова» ru Moscow State University ru ФГБОУ ВО «Московский государственный университет им. М.В. Ломоносова» ru Moscow State University ru 25 06 2016 25 06 2016 1 1 95 98 20 06 2016 20 06 2016 https://rusjbpc.ru/en/nauka/article/54002/view

Каталитическим центром кислород-выделяющего комплекса (КВК) фотосистемы 2 (ФС2) является кластер Mn4CaO5, структура которого в настоящее время расшифрована. Экстракция катионов кальция из КВК приводит к разобщению функции выделения молекулярного кислорода и электронного транспорта через ФС2 [Semin et al. Photosynth. Res. 98 (2008) 235] и одновременному появлению уширенного ЭПР сигнала с g = 2 (расщепленный «S3» сигнал). Не исключено, что это взаимосвязанные эффекты. Однако ЭПР сигнал «S3» наблюдается не только при удалении Ca2+ из КВК, но и после обработки нативных мембранных препаратов ФС2 анионами фтора, ацетата и хлоридом аммония. Ранее мы установили, что анионы фтора, подобно экстракции Ca2+, разобщают электрон-транспортные процессы и выделение кислорода. В представленной работе изучено действие хлорида и нитрата аммония на функциональную активность мембранных препаратов ФС2 с нативным КВК. Обнаружено, что как хлорид, так и нитрат аммония ингибирует выделение кислорода более эффективно, чем восстановление искусственного акцептора электронов 2,6-дихлорфенолиндофенола на акцепторной стороне ФС2, то есть действие аммония приводит к разобщению этих процессов. Предполагается, что ингибирующий эффект аммония связан с инактивацией функции Са2+.

Catalytic center of oxygen-evolving complex (OEC) of photosystem II (PSII) is Mn4CaO5 cluster structure of which is determined now. Ca2+ extraction from OEC is accompanied by decoupling of oxygen evolution and electron transport processes in PSII and by the appearance of a broad EPR signal (so-called “S3” or “split signal”) at low temperatures. These data imply that decoupling and EPR effects can be interdependent. It is interesting that EPR signal «S3» is observed also after treatment of native PSII membranes by fluoride anions, acetate and chloride of ammonia. Previously we found that fluoride anions treatment of PSII like Ca2+ extraction from the OEC provide the appearance of decoupling effect. In the presented work we investigated the effect of ammonia chloride and ammonia nitrate on the functional activity of native PSII membranes to study the effect of anions. We found that ammonia nitrate like ammonia chloride inhibit the oxygen evolution more effectively than the reduction of artificial electron acceptor 2,6- dichlorophenolindophenol that is the action of ammonia decouples these processes. However the inhibition doesn’t depend on the anions. We suggest that ammonia inhibition effect is determined by inactivation of Ca2+ function.

 фотосистема 2 кислород-выделяющий комплекс аммоний photosystem II oxygen-evolving complex ammonia

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