Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54031 МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ STUDY OF THE DISTRIBUTION OF TRANSPOSABLE ELEMENTS IN THE GENOME LITTORINA SAXATILIS ИССЛЕДОВАНИЕ ПРЕДСТАВЛЕННОСТИ МОБИЛЬНЫХ ГЕНЕТИЧЕСКИХ ЭЛЕМЕНТОВ В ГЕНОМЕ LITTORINA SAXATILIS Пузаков М В Puzakov M V puzakov@ngs.ru Пузакова Л В Puzakova L V ФГБНУ Институт природно-технических систем ru Institute of Natural and Technical Systems ru ФГБНУ Институт природно-технических систем ru Institute of Natural and Technical Systems ru 25 06 2016 25 06 2016 1 1 211 215 20 06 2016 20 06 2016 https://rusjbpc.ru/en/nauka/article/54031/view

В данной работе была изучена представленность мобильных генетических элементов (МГЭ) в геноме морского брюхоногого моллюска Littorina saxatilis . Нами были исследованы нуклеотидные последовательности протяженностью около 360 тыс. пар нуклеотидов. В результате анализа в геноме L. saxatilis были обнаружены повторы, имеющие сходство как с ретротранспозонами, так и с ДНК-транспозонами. Количество выявленных ретротранспозонов превышало количество ДНК-транспозонов в два с половиной раза (77 и 30, соответственно), что согласуется с тем, что репликативный способ перемещения способствует накоплению МГЭ в геноме. Среди ретротранспозонов наиболее представлены элементы семейств Copia , Gypsy , CR1 и DIRS . У ДНК-транспозонов самыми многочисленными оказались семейства Mariner , hAT и Sola . Далее планируется изучить структуры обнаруженных повторов и определить какие из них являются потенциально функциональными МГЭ.

In this work it was studied the distribution of transposable elements (TE) in the genome of the marine gastropod Littorina saxatilis . We investigated the nucleotide sequence of a length of about 360 thousand bp. Genome analysis L. saxatilis has revealed the repeats that are similar to the retrotransposons and DNA-transposons. The number of detected retrotransposons was higher than the amount of DNA-transposons in two and a half times (77 and 30, respectively). This is consistent with the fact that the replicative way to move contributes to the accumulation of the TE in the genome. Among the most widely represented retrotransposons were members of families of Copia , Gypsy , CR1 and DIRS . Among the DNA transposons were the most numerous family of Mariner , hAT and Sola . Further, we going to study the structure of the detected repetition to determine which ones are potentially functional TE.

 ДНК мобильные генетические элементы стрессовая индукция инсерционный мутагенез Littorina saxatils DNA transposable elements stress induction insertional mutagenesis Littorina saxatilis

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