Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54047 МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ PROCESS OF DNA PACKAGING IN SOLUTIONS WITH POLYLYSINE AND ITS OLIGOPEPTIDES ПРОЦЕСС КОМПАКТИЗАЦИИ ДНК В РАСТВОРАХ, СОДЕРЖАЩИХ ПОЛИЛИЗИН И ЕГО ОЛИГОПЕПТИДЫ Касьяненко Н А Kasyanenko N A n.kasyanenko@spbu.ru Дрибинский Б А Dribinsky B A Санкт-Петербургский государственный университет ru Saint Petersburg State University ru Санкт-Петербургский государственный университет ru Saint Petersburg State University ru 25 06 2016 25 06 2016 1 1 273 277 20 06 2016 20 06 2016 https://rusjbpc.ru/en/nauka/article/54047/view

Проводится сравнение конденсации ДНК в растворе, содержащем поли-L-лизин с различным числом мономеров n = 2, 3, 5, 325 в присутствии низкомолекулярной соли 5 мМ NaCl. Было показано, что, несмотря на идентичные результаты конденсации ДНК, индуцированной полилизином и его олигомерами, кинетика компактизации ДНК и конформационные изменения макромолекулы до ее конденсации зависят от того, принадлежат ли конденсирующие агенты к высокомолекулярным поликатионам или коротким олигомерам. Вторичная и третичная структуры ДНК были изучены с помощью спектральных методов (сектрофотометрия, флуоресцентрная спектроскопия, круговой дихроизм), а также с использованием динамического рассеяния света, низкоградиентной вискозиметрии, двулучепреломления в потоке и атомной силовой микроскопии. В последнем случае изображения были получены после фиксации молекул ДНК из раствора на поверхность слюды в присутствии ионов магния.

A comparison of DNA condensation in a solution containing the poly-l-lysine with the different number of monomers n = 2, 3, 5, 270, 325 was carried out in the presence of low molecular electrolyte 5 mM NaCl. It was shown that despite the identical results of DNA condensation induced by compounds (poly-L-lysine and its oligopeptides), the kinetic of DNA packaging and conformational changes prior to condensation depends on whether they belong to a long polycations or short oligomers. DNA secondary and tertiary structures were examined using Spectral methods (Spectrophotometry, Fluorescent Spectroscopy, Circular Dichroism), and with Dynamic Light Scattering, Low Gradient Viscometry, Flow Birefringence, and AFM. AFM images were obtained after DNA fixation from solutions on mica surface with Mg ions.

 конденсация ДНК поли-L-лизин олигопептиды кинетика компактизации ДНК DNA condensation poly-L-lysine oligopeptides kinetic of DNA packaging

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