Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54070 БИООРГАНИЧЕСКАЯ, БИОФИЗИЧЕСКАЯ И МЕДИЦИНСКАЯ ХИМИЯ BIOORGANIC, BIOPHYSICAL AND MEDICINAL CHEMISTRY БИООРГАНИЧЕСКАЯ, БИОФИЗИЧЕСКАЯ И МЕДИЦИНСКАЯ ХИМИЯ POLYELECTROLYTE SYSTEM DELIVERY USING MUCIN ПОЛИЭЛЕКТРОЛИТНАЯ СИСТЕМА ДОСТАВКИ ЛЕКАРСТВЕННЫХ ВЕЩЕСТВ С ИСПОЛЬЗОВАНИЕМ МУЦИНА Шолина Е А Sholina E A Филатова Л Ю Filatova L Y Киржанова Е А Kirzhanova E A Балабушевич Н Г Balabushevich N G nbalab2008@gmail.com Московский государственный университет им. М.В. Ломоносова ru Lomonosov Moscow State University ru Московский государственный университет им. М.В. Ломоносова ru Lomonosov Moscow State University ru Московский государственный университет им. М.В. Ломоносова ru Lomonosov Moscow State University ru Московский государственный университет им. М.В. Ломоносова ru Lomonosov Moscow State University ru 25 12 2016 25 12 2016 1 2 82 86 20 12 2016 20 12 2016 https://rusjbpc.ru/en/nauka/article/54070/view

Мукозальное применение биологически активных веществ, в том числе высокомолекулярных веществ, интенсивно развивается последние десятилетия. Полиэлектролитная система доставки лекарственных веществ получена путем послойной адсорбции муцина и протамина на микросферах ватеритной формы карбоната кальция. Условия процесса влияли как на включение муцина в процессе получения микросфер СаСО3, так и на его адсорбцию на мезопористой поверхности частиц. Изучены стабильность, морфология, изменение поверхностного заряда частиц при попеременной адсорбции на микросферах СаСО3 отрицательно заряженного муцина и положительно заряженного протамина. Проведен подбор условий растворения ядер СаСО3 в готовых полиэлектролитных микрочастицах. Обсуждены три способа иммобилизации биологически активных веществ (преимущественно белков, ферментов, пептидов) в полиэлектролитную систему на основе муцина, а также перспективность ее использования для доставки биологически активных веществ через слизистые оболочки.

Mucosal applications for different biologically active ingredients including high molecular weight substances have been developed actively last decades. Polyelectrolyte drug delivery system was obtained by mucin and protamine layer-by-layer (LbL) adsorption on microspheres made of calcium carbonate vaterite form. Process conditions influenced both mucin entrapment during CaCO3 microspheres formation and its further adsorption on mesoporous surface of the particles. Particle stability, morphology, surface charge during negatively charged mucin and positively charged protamine LbL adsorption on CaCO3 microspheres have been studied. Conditions for CaCO3-core dissolution inside formed LbL-particles have been selected. Three different ways for biologically active substances (mostly proteins, enzymes and peptides) immobilization in polyelectrolyte mucin-based system have been discussed together with their possible mucosal application.

 муцин адсорбция микросферы СаСO3 протамин послойная адсорбции полиэлектролитов мукоадгезивная система доставки лекарственных веществ mucin adsorption CaCO3-microspheres protamine polyelectrolyte layer-by-layer adsorption mucoadhesive drug delivery system

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