Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54179 МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ MOLECULAR BIOPHYSICS AND PHYSICS OF BIOMOLECULES МОЛЕКУЛЯРНАЯ БИОФИЗИКА И ФИЗИКА БИОМОЛЕКУЛ The role of beta-casein structural state in binding and solubilization of retinol Роль структурного состояния бета-казеина в его связывающих и солюбилизационных свойствах по отношению к ретинолу Коннова Т А Konnova T A tatiana.a.konnova@gmail.com Файзуллин Д А Faizullin D A Зуев Ю Ф Zuev Yu F Казанский институт биохимии и биофизики Казанского научного центра РАН ru Kazan Institute of Biochemistry and Biophysics KazSC RAS ru Казанский институт биохимии и биофизики Казанского научного центра РАН ru Kazan Institute of Biochemistry and Biophysics KazSC RAS ru Казанский институт биохимии и биофизики Казанского научного центра РАН ru Kazan Institute of Biochemistry and Biophysics KazSC RAS ru 25 06 2017 25 06 2017 2 1 309 313 20 06 2017 20 06 2017 https://rusjbpc.ru/en/nauka/article/54179/view

Использование комплекса взаимодополняющих биофизических методов (флуоресцентная, КД- и ИК-спектроскопия, динамическое светорассеяние) позволило изучить корреляцию между агрегатным состоянием (мономеры, ассоциаты), реологическими свойствами белка и вторичной структурой природной и рекомбинантных форм бета-казеина. Получен массив экспериментальных данных о механизмах самоассоциации бета-казеина, уточняющий границы его коллоидного состояния, определены температуры переходов мономер-мицелла, размеры агрегатов природной и модифицированных форм белка. Охарактеризована мицеллообразующая способность и солюбилизационная емкость мицелл бета-казеина по отношению к гидрофобному витамину ретинолу. Проанализировано влияние ионной силы раствора на связывание и термодинамическую стабильность комплексов бета-казеина с гидрофобным лигандом. Рассчитанные константы связывания подтверждают, что связывание в комплексах ретинол-бета-казеин зависит от конформации белковой молекулы и ионной силы раствора. Полученные представления о механизмах конформационных переходов, самоассоциации, взаимодействия бета-казеина с целевыми лигандами на молекулярном уровне, способствуют разработке и созданию эффективных белковых носителей биоактивных добавок, лекарственных препаратов.

The correlation between the aggregate state (monomers, associates), rheological properties and the secondary structure of the native and recombinant beta-caseins was studied by the complex of biophysical methods (fluorescence, CD and IR spectroscopy, dynamic light scattering). The experimental data on the mechanisms of beta-casein self-association that specifies its colloidal state limits was obtained, the temperature of monomer-micelle transitions, the size of aggregates of protein native and modified forms were determined. Micelle-forming ability and micellar solubilization capacity of beta-casein micelles towards hydrophobic vitamin retinol were characterized. The effect of the low ionic strength of the solution on the binding and thermodynamic stability of beta-casein complexes with a hydrophobic ligand was analyzed. The resulting binding constants confirm that the binding in retinol-beta-casein complexes depends on the conformation of the protein molecule and the ionic strength of the solution. The obtained insights on the mechanisms of conformational transitions, self-association, interaction of beta-casein with target ligands at the molecular level, contribute to the design and development of effective protein carriers of bioactive additives and drugs.

 бета-казеин мицеллы самоассоциация критическая концентрация мицеллообразования константа связывания beta-casein micelle self-association critical micelle concentration (CMC) binding constant

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