Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54244 Общая биофизика General biophysics Общая биофизика CELL SEPARATION BY BARRIER CONTACTLESS DIELECTROPHORESIS ВОЗМОЖНОСТИ СЕПАРАЦИИ КЛЕТОК МЕТОДОМ БЕСКОНТАКТНОГО БАРЬЕРНОГО ДИЭЛЕКТРОФОРЕЗА Сорокина О Н Sorokina O N alsiona@gmail.com Подойницын С Н Podoynitsyn S N Климов М А Klimov M A Левин И И Levin I I Симакин С Б Simakin S B Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics of RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics of RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics of RAS ru ООО «ИОНТЕК-нано» ru JSC «IONTEC-nano» ru ООО «ИОНТЕК-нано» ru JSC «IONTEC-nano» ru 25 03 2018 25 03 2018 3 1 51 59 20 03 2018 20 03 2018 https://rusjbpc.ru/en/nauka/article/54244/view

Предложена конструкция сепарационного устройства и метод разделения микробиологических образцов в устройствах, реализующих барьерный бесконтактный диэлектрофорез. Принцип работы сепарационного устройства основан на формировании диэлектрофоретических барьеров в объеме сепарационной камеры, благодаря чему разрабатываемый метод отличается высокой производительностью и простотой использования по сравнению с традиционными планарными системами диэлектрофореза. Кроме того, особенностью метода можно назвать отсутствие контакта разделяемых образцов с проводящими элементами электродной структуры. Проведено компьютерное моделирование распределения напряженностей электрических полей в области формирования диэлектрофоретических барьеров, а также показано влияние толщины изолирующего покрытия на диэлектрофоретические свойства барьеров. На основании данных компьютерного моделирования проведены оценки сил, действующих на частицу (клетку дрожжей диаметром 7 мкм) в сепарационной камере. Теоретически предсказана и экспериментально подтверждена возможность задерживания клеток на ДЭФ барьерах на примере дрожжевых грибов Saccharomyces cerevisiae.

A design of a separation device and a method for microbiological samples separation based on barrier contactless dielectrophoresis are proposed. The principle of operation of the separation device is based on the formation of dielectrophoretic barriers in the volume of the separation chamber. The suggested method is highly efficient and easy to use in comparison with traditional planar dielectrophoresis systems. The feature of the method is the absence of contact between the separated samples and the conductive elements of the electrode structure. A computer simulation of the distribution of electric field over the dielectrophoretic barriers was carried out, and the effect of the thickness of the insulating coating on the dielectrophoretic properties of the barriers was shown. Based on the computer simulation data, the forces acting on the particle (a yeast cell with a diameter of 7 μm) in the separation chamber were estimated. The possibility of cell retention on cDEP barriers is theoretically predicted and experimentally confirmed using the example of yeast Saccharomyces cerevisiae .

 сепарация клеток бесконтактный барьерный диэлектрофорез cell separation barrier contactless dielectrophoresis

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