Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54259 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ DETERMINATION OF BIOLOGICALLY ACTIVE VOLATILE ORGANIC COMPOUNDS BY SOLID-PHASE MICROEXTRACTION ОПРЕДЕЛЕНИЕ БИОЛОГИЧЕСКИ АКТИВНЫХ ЛЕТУЧИХ ОРГАНИЧЕСКИХ СОЕДИНЕНИЙ ТВЕРДОФАЗНОЙ МИКРОЭКСТРАКЦИЕЙ Мишарина Т А Misharina T A tmish@rambler.ru Теренина М Б Terenina M B Крикунова Н И Krikunova N I Институт биохимической физики им. Н.М. Эмануэля РАН; Российский экономический университет им. Г.В. Плеханова ru Emanuel Institute of Biochemical Physics, Russian Academy of Sciences; Plekhanov Russian Economic University ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics, Russian Academy of Sciences ru Институт биохимической физики им. Н.М. Эмануэля РАН ru Emanuel Institute of Biochemical Physics, Russian Academy of Sciences ru 25 03 2018 25 03 2018 3 1 146 152 20 03 2018 20 03 2018 https://rusjbpc.ru/en/nauka/article/54259/view

Метод твердофазной микроэкстракции в сочетании с капиллярной газожидкостной хроматографией использован для изучения состава биологически активных компонентов смесей эфирных масел, присутствующих в газовой фазе над образцом. Найдено, что эффективность извлечения летучих веществ зависела от состава образца, концентрации эфирных масел, от свойств и структуры летучих соединений, от природы полимерного сорбента, от времени извлечения. При увеличении концентрации исследуемых образцов эфирных масел увеличивалась сорбция более летучих неполярных монотерпенов и уменьшалась сорбция высококипящих ацетатов и сесквитерпенов. Максимальной сорбционной активностью и емкостью обладал полидиметилсилоксан, минимальной - смешанный полимер полидиметилсилоксана с карбоксеном. Найдено, что добавление воды к смеси эфирных масел приводило к увеличению экстракции неполярных соединений и уменьшению сорбции полярных веществ.

The solid-phase microextraction method in combination with capillary gas-liquid chromatography was used to study the composition of the biologically active components of a mixture of essential oils present in the gas phase above the sample. It was found that the extraction efficiency of volatile substances depended on the composition of the sample, the concentration of essential oils, the properties and structure of volatile compounds, the nature of the polymer sorbent, and the extraction time. The increase in concentration of essential oils in test samples resulted in the increase of sorption of more volatile nonpolar monoterpenes and decrease of sorption of high-boiling acetates and sesquiterpenes. It was found that polydimethylsiloxane had the highest sorption activity and capacity, a mixed polymer of polydimethylsiloxane with carboxene showed the minimal values. The addition of water to a mixture of essential oils led to an increase in the extraction of nonpolar compounds and a decrease in the sorption of polar substances.

 твердофазная микроэкстракция капиллярная газожидкостная хроматография летучие органические соединения эфирные масла solid-phase microextraction capillary gas-liquid chromatography volatile organic compounds essential oils

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