Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54269 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ ALUMINUM IONS INFLUENCE ON CHANGES OF SPECTRAL AND FLUORESCENT PROPERTIES OF AMYLOID FIBRILLS FROM LYSOZYME ВЛИЯНИЕ ИОНОВ АЛЮМИНИЯ НА ИЗМЕНЕНИЯ СПЕКТРАЛЬНО-ФЛУОРЕСЦЕНТНЫХ ХАРАКТЕРИСТИК АМИЛОИДНЫХ ФИБРИЛЛ, ПОЛУЧЕННЫХ ИЗ ЛИЗОЦИМА Скоробогатова А С Skarabahatava A S sas.alesya@gmail.com Венская Е И Venskaya E I Зубрицкая Г П Zubritskaya G P Лукьяненко Л М Lukyanenko L M Слобожанина Е И Slobozhanina E I Институт биофизики и клеточной инженерии НАН Беларуси ru Institute biophysics and cell engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute biophysics and cell engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute biophysics and cell engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute biophysics and cell engineering of NAS of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute biophysics and cell engineering of NAS of Belarus ru 25 03 2018 25 03 2018 3 1 204 208 20 03 2018 20 03 2018 https://rusjbpc.ru/en/nauka/article/54269/view

Изучено влияние ионов алюминия в концентрации 100 мкМ на рост амилоидных структур из лизоцима. При использовании флуоресцентного зонда тиофлавина было обнаружено, что добавление ионов алюминия в среду роста амилоидных фибрилл из лизоцима вызывает увеличение интенсивности флуоресценции используемого зонда, что говорит о более высокой скорости образования амилоидных фибрилл при добавлении ионов металла. При этом использование другого флуоресцентного зонда - 1-анилино-8-нафталинсульфоната (АНС) не позволило обнаружить структурных различий амилоидных фибрилл с и без хлорида алюминия в среде инкубации. Также нами была изучена собственная тирозиновая и триптофановая флуоресценция амилоидных фибрилл на основе лизоцима и их комплекса с ионами алюминия. Показано, что добавление в среду роста амилоидных фибрилл 100 µМ хлорида алюминия приводит к снижению интенсивности собственной флуоресценции по сравнению с амилоидными фибриллами, полученными только на основе лизоцима.

Influence of 100 µM aluminum ions on amyloids fibril growth from lysozyme was studied. Using fluorescent probe thioflavin T it was shown that addition aluminum ions to growth medium of amyloid fibrils from lysozyme leads to increasing fluorescent intensity of applied probe. This suggests that aluminum ions stimulate formation of amyloid fibrils. However, applying another fluorescent probe - 1-anilinonaphthalene 8-sulfonate (ANS) we couldn’t find structural differences between amyloid fibril with and without aluminum chloride in the environment. Also we studied own fluorescent of tyrosine and tryptophan of amyloid fibrils from lysozyme ad their complexes with aluminum ions. It was shown that 100 µM aluminum ions lead to decreasing intrinsic fluorescence in compare with amyloid fibrils only from lysozyme.

 амилоидные структуры лизоцим хлорид алюминия флуоресцентные зонды amyloid structures lysozyme aluminum chloride fluorescent probes

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